| 商品蛋鸡成髓细胞瘤、血管瘤型J亚群白血病病毒特性的研究 |
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| 引用本文： | 孟祥凯,刘青,王海伦,张洪海,邱波,刘功振,成子强.商品蛋鸡成髓细胞瘤、血管瘤型J亚群白血病病毒特性的研究[J].畜牧兽医学报,2008,39(11). |
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| 作者姓名： | 孟祥凯 刘青 王海伦 张洪海 邱波 刘功振 成子强 |
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| 作者单位： | 1. 山东农业大学动物科技学院,泰安,271018
2. 山东东营市水利局,东营,257091 |
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| 基金项目： | 山东省优秀中青年科学家研究奖励基金 |
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| 摘 要： | 2007年7月山东某蛋鸡场150日龄海兰褐蛋鸡发病，前期经病理学和免疫组织化学检测证明和ALV—J密切相关。取4只病鸡的肝组织处理后接种鸡胚成纤维细胞（CEF）培养，用ALV—J单抗G2-3进行间接免疫荧光检测，结果呈现强阳性，将反应为阳性的4株病毒分别命名为WS0701、WS0702、WS0703和WS0704；根据ALV-J原型株HPRS-103的序列设计1对针对外源性ALV—J的引物P1和P2，提取阳性CEF基因组DNA作为模板，PCR扩增后，得到长度为924bp的片段；PCR扩增产物测序结果显示，与HPRS-103的同源性为95．0％～97．5％，与国内分离株的同源性为92．0％～95．9％；遗传变异分析结果表明成髓细胞瘤型、血管瘤型ALV-J与国内毒株的同源性较远，而与HPRS-103的同源性最近，这说明ALV—J在蛋鸡体内复制的过程中有返祖现象，并呈现了新的肿瘤学特征。
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| 关 键 词： | 商品蛋鸡 J亚群白血病 免疫学 病毒学 |
Studies on Viral Characteristic of Myeloblastosis and Hemangioma Associated with ALV-J in Commercial Layer Chicken |
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| MENG Xiang-kai,LIU Qing,WANG Hai-lun,ZHANG Hong-hai,QIU Bo,LIU Gong-zhen,CHENG Zi-qiang.Studies on Viral Characteristic of Myeloblastosis and Hemangioma Associated with ALV-J in Commercial Layer Chicken[J].Acta Veterinaria et Zootechnica Sinica,2008,39(11). |
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| Authors: | MENG Xiang-kai LIU Qing WANG Hai-lun ZHANG Hong-hai QIU Bo LIU Gong-zhen CHENG Zi-qiang |
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| Institution: | MENG Xiang-kai1,LIU Qing1,WANG Hai-lun2,ZHANG Hong-hai1,QIU Bo1,LIU Gong-zhen1,CHENG Zi-qiang1(1.College of Animal Science , Veterinary Medicine,Sh,ong Agricultural University,Tai\'an 271018,China,2.Dongying Water Conservancy Bureau,Sh,ong Province,Dongying 257091,China) |
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| Abstract: | Myeloblastosis and hemangioma cases associated with avian leukosis virus subgroup J(ALV-J) were first diagnosed in a layer chicken flock in Shandong province on July 2007.The CEFs inoculated by virus extracted from liver were cultured,and detected by indirect immunofluorescence with ALV-J monoclonal antibody G2-3,the results were intense positive.The four isolated strains were named as WS0701,WS0702,WS0703 and WS0704.A pair of primers P1 /P2 was designed according to the sequence of prototype ALV-J HPRS-103... |
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| Keywords: | commercial layer chicken subgroup J of avian leukosis virus immunology virology |
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