| HPLC法测定六味地黄丸熊果酸的含量 |
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| 引用本文： | 卢翠文.HPLC法测定六味地黄丸熊果酸的含量[J].安徽农业科学,2011,39(4):2070-2071. |
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| 作者姓名： | 卢翠文 |
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| 作者单位： | 柳州师范高等专科学校,广西柳州,545004 |
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| 摘 要： | 目的]用高效液相色谱（HPLC）法测定六味地黄丸（浓缩丸）的熊果酸含量,提高熊果酸的质量控制标准。方法]色谱柱：WelchMaterials AQ-C18柱（5μm,4.6 mm×250.0 mm）;流动相：乙腈-水-磷酸（V∶V∶V=90∶10∶0.1）;柱温：25℃;流速：1.0 ml/min;检测波长为210 nm。结果]在0.457 6~1.542 3μg/ml的线性范围内,熊果酸与峰面积呈良好的线性关系,平均回收率为99.31%,RSD值为1.09%（n=6）。结论]高效液相色谱法灵敏、简单、快速、准确,适用于六味地黄丸（浓缩丸）熊果酸含量的测定。
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| 关 键 词： | 六味地黄丸（浓缩丸） 熊果酸 含量测定 高效液相色谱法 |
Determination of Ursolic Acid in Liuwei Dihuang Pills by HPLC |
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| LU Cui-wen.Determination of Ursolic Acid in Liuwei Dihuang Pills by HPLC[J].Journal of Anhui Agricultural Sciences,2011,39(4):2070-2071. |
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| Authors: | LU Cui-wen |
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| Institution: | LU Cui-wen(Liuzhou Teachers College,Liuzhou,Guangxi 545004) |
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| Abstract: | Objective] To determine the content of ursolic acid in liuwei dihuang pills by HPLC,so as to promote the quality control standard of ursoli acid.Method] HPLC conditions were as follows: column of Welch Materials AQ-C18 column(5 μm,4.6 mm×250.0 mm);mobile phase of acetonitrile-water-phosphoric acid(V∶ V∶ V=90∶ 10∶ 0.1);column temperature of 25 ℃;flow rate of 1.0 ml/min and detection wavelength of 210 nm.Result]The relationship between ursolic acid and peak area was good in the range of 0.457 6~1.542 3 μg/ml,and the mean recovery of ursolic acid was 99.31% with 1.09% in RSD value(n=6).Conclusion]Simple in operation,accurate in result,HPLC method is favorable for the determination of ursolic acid in liuwei dihuang pills. |
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| Keywords: | Liuwei dihuang pills(concentration pills) Ursolic acid Determination of content HPLC method |
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