| 共轭亚油酸高产菌株的筛选及培养基的优化 |
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| 引用本文： | 罗玉芬,徐尔尼,巫小丹.共轭亚油酸高产菌株的筛选及培养基的优化[J].安徽农业科学,2011,39(4):2289-2291. |
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| 作者姓名： | 罗玉芬 徐尔尼 巫小丹 |
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| 作者单位： | 南昌大学生命科学与食品工程学院,食品科学与技术国家重点实验室,江西南昌,330047 |
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| 摘 要： | 目的]从自然发酵的酸菜汁中筛选共轭亚油酸高产菌株以及优化该菌株的培养基。方法]以3种自然发酵的泡菜为研究对象,通过溴甲酚紫平板筛选产酸细菌,革兰氏染色法初步鉴定,紫外吸收光谱法检测发酵液中共轭亚油酸含量等方法,从酸菜汁中筛选到一株共轭亚油酸高产菌株QL2,对其培养基成分碳源、氮源和无机盐离子及底物亚油酸的添加量进行优化。结果]菌株QL2共轭亚油酸产量达23.263μg/ml,亚油酸转化率为3.88%。优化后的培养基组成为：乳糖20 g/L,酵母膏30 g/L,CH3COONa2 g/L,MgSO4.7H2O0.8 g/L,K2HPO4.3H2O3 g/L。优化培养基组成成分后,底物亚油酸添加量为0.40%（V/V）时,共轭亚油酸产量达288.740μg/ml,转化率高达7.21%。结论]研究为产共轭亚油酸菌株的筛选及培养基的优化提供了参考。
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| 关 键 词： | 共轭亚油酸 高产菌株 筛选 培养基 |
Research on the Screening of High CLA-producing Strain and the Optimization of Its Medium |
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| LUO Yu-fen et al.Research on the Screening of High CLA-producing Strain and the Optimization of Its Medium[J].Journal of Anhui Agricultural Sciences,2011,39(4):2289-2291. |
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| Authors: | LUO Yu-fen |
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| Institution: | LUO Yu-fen et al(State Key Laboratory of Food Science and Technology,School of Life Sciences and Food Engineering,Nanchang University,Nanchang,Jiangxi 330047) |
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| Abstract: | Objective] The high conjugated linoleic acid(CLA)-producing strain from natural fermented pickled cabbage pickle juice was screened and its medium was optimized.Method] Three kinds of natural fermented pickled cabbage juices being taken as experimental material,the bacterial strain producing acid was screened with the method of bromocresol purple plate and one high CLA-producing strain-QL2 was obtained after the content of the CLA was measured through the preliminary identification of Gram staining and UV... |
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| Keywords: | Conjugated linoleic acid High CLA-producing strain Screen Medium |
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