| 不同基因型杜仲叶片中绿原酸含量的检测 |
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| 引用本文： | 赵静,杨俊霞,焦永康,李蕾,郭宝林.不同基因型杜仲叶片中绿原酸含量的检测[J].安徽农业科学,2011,39(4):2103-2104. |
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| 作者姓名： | 赵静 杨俊霞 焦永康 李蕾 郭宝林 |
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| 作者单位： | 赵静,杨俊霞,李蕾,郭宝林(河北农业大学林学院,河北保定,071000);焦永康(河北农业大学植保学院,河北保定,071000) |
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| 摘 要： | 目的]检测不同基因型杜仲叶片中的绿原酸含量。方法]以超声波助提法提取绿原酸,采用HPLC法检测和二倍体含量,色谱柱为Eclipse xdb-c18柱（4.6 mm×150.0 mm,5μm）,以乙腈-0.4%磷酸水（10∶90）为流动相,检测波长为327 nm。结果]四倍体和二倍体杜仲叶片中绿原酸的平均含量分别为4.005和为0.850 mg/g。结论]四倍体杜仲叶片中绿原酸含量显著高于二倍体。
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| 关 键 词： | 杜仲叶 绿原酸 高效液相色谱 含量 不同基因型 |
Determination of Chlorogenic Acid in Eucommia Ulmoides of Different Genotypes |
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| ZHAO Jing et al.Determination of Chlorogenic Acid in Eucommia Ulmoides of Different Genotypes[J].Journal of Anhui Agricultural Sciences,2011,39(4):2103-2104. |
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| Authors: | ZHAO Jing |
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| Institution: | ZHAO Jing et al(College of Forestry,Agricultural University of Hebei,Baoding,Hebei 071000) |
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| Abstract: | Objective]Chlorogenic acid content in eucommia ulmoides of different genotypes were detected.Method]Ultrasonic was used to extract chorogenic acid.The content of Chlorogenic acid in Eucommia ulmoides at different genotraction was determined by HPLC method.The chromatographic column was xdb-c18(4.6 mm × 150.0 mm,5 μm) and the mobile phase consisted of acetonit rile-0.4% H3PO4 solution(10∶ 90) with a detection of 327 nm wavelength.Result]The results showed that average content of chlorogenic in tetraploid eucommia ulmoides was 4.005 mg/g,and average of chlogenic in diploid eucommia ulmoides was 0.850 mg/g.Conclusion]Multiple comparison found that the tetraploid eucommia ulmoides of chlorogenic acid were significantly higher than the diploid content of chlorogenic acid in leaves. |
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| Keywords: | Eucommia leaves Chlorogenic acid HPLC Content Different genotypes |
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