Genetic diversity of wheat wild relatives in the Near East detected by AFLP |
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| Authors: | Tsuneo Sasanuma Kamel Chabane Takashi R Endo Jan Valkoun |
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| Institution: | (1) Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan;(2) Genetic Resources Unit, International Center for Agricultural Research in the Dry Areas, P.O. Box 5466, Aleppo, Syria |
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| Abstract: | In order to reveal the molecular genetic diversity of wheat wild relatives, an AFLP analysis was conducted with 16 accessions
of five Triticum andAegilops species originating from the Near East. Variation within population was studied with at least seven individuals per accession.
Four primer combinations were used for selective amplification. Based on the scored bands, we estimated percentage of polymorphic
bands, 1 – proportion of shared bands (1-psb) and nucleotide diversity (π). Of the five species used in this study, Ae.
speltoides had the highest level of `within population' variation. This species had also the highest value of the variation among populations.
As for Triticum species, the level of variation within population was low in diploid species (T. urartu and T. boeoticum),whereas two tetraploid species (T. dicoccoides and T. araraticum) had relatively high levels of variation within population. While the two diploid Triticum indicated a clear interspecific divergence, the two tetraploid wild wheats were not clearly divergent in this study. The
variance portioning analysis indicated that the variation detected for diploid Triticum species was mainly composed of `between species' variation, on the other hand that for tetraploid Triticum was mostly composed of `within population' variation. In conclusion, AFLP analysis reveals molecular variation in all accessions
used in this study, suggesting a potential genetic diversity of the wheat wild relatives in natural populations. These results
have implications for the design of strategies to maintain genetic diversity within genebank collections.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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| Keywords: | Ae speltoides AFLP geneticdiversity the Near East wild wheats |
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