| 紫贻贝4种同工酶的组织特异性表达研究 |
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| 引用本文： | 刘慧慧,CHANG Kangmei.紫贻贝4种同工酶的组织特异性表达研究[J].南方水产,2008,4(4):60-63. |
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| 作者姓名： | 刘慧慧 CHANG Kangmei |
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| 作者单位： | 浙江海洋学院海洋科学学院,浙江,舟山,316004 |
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| 基金项目： | 浙江省自然科学基金，浙江省科技攻关项目，浙江海洋学院校内项目 |
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| 摘 要： | 采用聚丙烯酰胺垂直电泳法对紫贻贝Mytilu sedulis的鳃、消化腺、外套膜、足、闭壳肌等5种组织的超氧化物歧化酶（SOD）、三磷酸腺苷酶（ATPase）、苹果酸脱氢酶（MDH）、苹果酸酶（ME）进行了检测分析。结果表明，SOD在紫贻贝的5种组织中均有表达，共有4条酶带，但迁移率最大的酶带在足和外套膜中未出现，而在另外3种组织中呈弱表达；ATPase在除消化腺外的组织中都表现为2条带；MDH在5种组织中都表达出1条酶带，其中足和闭壳肌的MDH染色最深；ME存在1～2条酶带，其中迁移率大的条带在5种组织中都有表达，而迁移率小的条带只存在于外套膜、闭壳肌和足中。从检测结果来看，上述4种酶在紫贻贝体内的表达有一定的组织特异性．
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| 关 键 词： | 紫贻贝 同工酶 组织特异性 |
Study on tissue-specificity expression of 4 isozymes in Mytilus edulis |
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| LIU Huihui,CHANG Kangmei.Study on tissue-specificity expression of 4 isozymes in Mytilus edulis[J].South China Fisheries Science,2008,4(4):60-63. |
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| Authors: | LIU Huihui CHANG Kangmei |
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| Institution: | (Marine Science College, Zhefiang Ocean University, Zhoushan 316004, China) |
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| Abstract: | SOD, ATPase, MDH and ME isoenzymes in gill, digestive gland, foot, adductor muscle and mantle of Mytilus edulis were tested and analyzed using polyacrylamide gel electrophoresis （PAGE）. The results indicated that four SOD bands were identified in five tissues, and the band with the biggest mobility ratio were found weakly in gill, digestive gland and adductor muscle but not in foot and mantle. Two ATPase bands were found in four tissues except digestive gland. One MDH band was found in five tissues, but the MDH gene expressed strongly in foot and adductor muscle. Two ME bands were examined in foot, adductor muscle and mantle, but the band with smaller mobility ratio was found in gill and digestive gland. Four isozymes showed diverse bands in different tissues, displaying tissue-specificity |
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| Keywords: | Mytilus edulis isozyme tissue-specificity |
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