| 分光光度法测定两种蟹类精子密度的研究 |
| |
| 引用本文： | 管卫兵,杨牧川,王成辉.分光光度法测定两种蟹类精子密度的研究[J].鲑鳟渔业,2012(1):35-37,57. |
| |
| 作者姓名： | 管卫兵 杨牧川 王成辉 |
| |
| 作者单位： | [1]上海海洋大学海洋科学学院,上海201306 [2]上海海洋大学水产与生命学院,上海201306 |
| |
| 基金项目： | 基金项目：上海市中华绒螯蟹产业技术体系建设（2010-2014） |
| |
| 摘 要： | 蟹类精子计数对其冷冻保存及种群生殖能力的探究有重要意义。为了提高计数蟹类精子数量的速度,建立了分光光度法测定蟹类精子密度的方法。比较了两种蟹类在不同波长（380nm、520nm、760nm）下吸光度（A）与精子密度（C）的关系。结果表明,在520nm波段下三疣梭子蟹（Portunus trituberculatus）精子密度和吸光度之间也存在线性回归关系,其回归方程是：A=0.058×C（R2=0.9928）;中华绒螯蟹（Eriocheir sinensis）精子吸光度与精子密度呈线性回归关系,其回归方程是：A=0.061×C（R2=0.9892）。统计检验表明,使用该方法测定蟹类精子数目,是一种快速而准确的方法,可以加速蟹类精子计数速度。
|
| 关 键 词： | 三疣梭子蟹 中华绒螯蟹 分光光度法 精子密度 |
Measurement of Sperm Concentration of Two Crab Species by Means of Spectrophotometric Method |
| |
| Authors: | GUAN Wei-bing YANG Mu-chuan WANG Cheng-hui |
| |
| Institution: | 1.College of Marine Sciences,Shanghai Ocean University,Shanghai 201306,China; 2.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China) |
| |
| Abstract: | It is significant of rapid measurement of crab sperm counts to study sperm cryopreservation and population reproduction.A spectrophotomeric method was used to determine the sperm concentration of swimming crab Portuns trituberculatus and Chinese mitten crab Eriocheir sinensis at wavelengths of 380,520,and 760 nm.It was found that the sensitive and reliable rapid measurement of crab sperm counts was carried out at 520 nm.A linear relationship between sperm concentration（C） and photometric absorbance（A） was observed for sperm concentration of swimming crab and Chinese mitten crab.The best regression equation for the standard curve at 520 nm was expressed as A= 0.058×C（R2=0.9928） for the swimming crab and A=0.061×C（R2=0.9892） for Chinese mitten crab.Statistical analysis showed that this spectrophotomeric method was fast and accurate for sperm count. |
| |
| Keywords: | Portuns trituberculatus Eriocheir sinensis spectrophotomeric method sperm concentration |
| 本文献已被 维普 等数据库收录！ |
|
