Serotype- and serogroup-specific detection of African horsesickness virus using phage displayed chicken scFvs for indirect double antibody sandwich ELISAs |
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Authors: | Wouter van Wyngaardt Cordelia Mashau Isabel Wright Jeanni Fehrsen |
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Institution: | 1Immunology Division, Onderstepoort Veterinary Institute, Private Bag X05, Onderstepoort 0110, South Africa.;2Deltamune, P. O. Box 14167, Lyttelton 0140, South Africa. |
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Abstract: | There is an ongoing need for standardized, easily renewable immunoreagents for detecting African horsesickness virus (AHSV). Two phage displayed single-chain variable fragment (scFv) antibodies, selected from a semi-synthetic chicken antibody library, were used to develop double antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISAs) to detect AHSV. In the DAS-ELISAs, the scFv previously selected with directly immobilized AHSV-3 functioned as a serotype-specific reagent that recognized only AHSV-3. In contrast, the one selected with AHSV-8 captured by IgG against AHSV-3 recognized all nine AHSV serotypes but not the Bryanston strain of equine encephalosis virus. Serving as evidence for its serogroup-specificity. These two scFvs can help to rapidly confirm the presence of AHSV while additional serotype-specific scFvs may simplify AHSV serotyping. |
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Keywords: | African horsesickness virus double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) phage-displayed scFv serotype-specific |
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