| 采用16S rDNA鉴定甜瓜细菌性叶斑病菌 |
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| 引用本文： | 李亚利,贾迎春,蒲崇建,杨成德,陈秀蓉.采用16S rDNA鉴定甜瓜细菌性叶斑病菌[J].植物保护,2007,33(3):65-68. |
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| 作者姓名： | 李亚利 贾迎春 蒲崇建 杨成德 陈秀蓉 |
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| 作者单位： | 甘肃农业大学草业学院 兰州730070(李亚利，杨成德，陈秀蓉)，甘肃省植保植检总站 兰州730030(贾迎春，蒲崇建) |
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| 基金项目： | 甘肃省科技厅科技攻关项目 |
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| 摘 要： | 从甘肃河西、新疆阿勒泰甜瓜上分离获得的2株致病细菌,通过16S rDNA序列测定以及序列同源性比较,结合病原菌落培养性状、菌体形态观察和革兰氏染色反应等,初步确定当地甜瓜细菌性叶斑病菌为丁香假单胞杆菌Pseudomonas syringae pv.lachrymans (Smith et Bryan) Younget al.]
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| 关 键 词： | 细菌性叶斑病 致病性测定 16S rDNA鉴定 |
| 修稿时间： | 2007-01-09 |
Identification of the pathogen of bacterial spot of cucurbits leaves based on 16S rDNA sequences |
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| Li Yali;Jia Yingchun;Pu Chongjian;Yang Chengde;Chen Xiurong.Identification of the pathogen of bacterial spot of cucurbits leaves based on 16S rDNA sequences[J].Plant Protection,2007,33(3):65-68. |
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| Authors: | Li Yali;Jia Yingchun;Pu Chongjian;Yang Chengde;Chen Xiurong |
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| Institution: | 1. College of Pratacultural Science, Gansu Agricultural University, Lanzhou 730070, China; 2. Plant Protection and Quarantine Station of Gansu , Lanzhou 730070, China |
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| Abstract: | Two strains of pathogenic bacteria were isolated in Hexi in Gansu and Aletai in Xinjiang.The strains were examined using 16S rDNA gene sequences.The results showed that the pathogens might be Pseudomonas syringae pv.lachrymans(Smith et Bryan) Young et al.,based on their culture patterns,morphological characteristics and staining reactions. |
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| Keywords: | bacterial leave spot pathogenicity identification based on 16S rDNA |
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