| 新疆雪莲芽分化过程蛋白质·可溶性糖含量及淀粉酶活性变化 |
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| 引用本文： | 徐春明,赵兵.新疆雪莲芽分化过程蛋白质·可溶性糖含量及淀粉酶活性变化[J].安徽农业科学,2009,37(3). |
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| 作者姓名： | 徐春明 赵兵 |
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| 作者单位： | 北京工商大学化学与环境工程学院,北京市植物资源研究开发重点实验室,北京;中国科学院过程工程研究所,生化工程国家重点实验室,北京,100080 |
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| 摘 要： | 目的]研究新疆雪莲分化过程中的生理生化变化。方法]将以雪莲无菌苗的叶片为外植体诱导的雪莲胚性愈伤组织置于MS+2 mg/L NAA+0.5 mg/L 6-BA(pH值为5.85~5.90)培养基上进行培养,对培养过程中雪莲细胞可溶性蛋白含量、可溶性糖、淀粉酶等参数进行测定分析。结果]在新疆雪莲整个培养阶段,胚性愈伤组织中的蛋白质含量高于非胚性愈伤组织,可溶性糖保持稳定,仅在芽分化阶段有一定的上升,α-淀粉酶活性保持稳定。胚性愈伤组织中的β-淀粉酶的活性均高于非胚性愈伤组织,这说明β-淀粉酶与细胞的分化过程密切相关。β-淀粉酶在整个培养过程中呈现双峰曲线。第1个峰出现在培养的开始阶段,第2个峰出现在芽分化时期。结论]该研究对研究新疆雪莲的高频再生具有重要的指导意义。
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| 关 键 词： | 新疆雪莲 愈伤组织 蛋白质 可溶性糖 淀粉酶 |
Study on the Change of Protein and Soluble Sugar Contents and Amylase Activity of Xinjiang Saussurea Iinvolucrata during Bud Differentiation |
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| Abstract: | Objective] The aim was to study the change of physiology and biochemistry of Xinjiang Saussurea involucrata during differentiation.Method] The embryogenic callus of S.involucrata induced by the explant that was from the leaves of S.involucrata aseptic seedlings was put on the medium of MS+2 mg/L NAA+0.5 mg/L 6-BA(pH 5.85-5.90)to culture,the parameters of soluble protein content,soluble sugar and amylase etc.of S.involucrata cells during culture process were determined and analyzed.Result] In the whole culture process of Xinjiang S.involucrata,the protein content of embryonic callus were higher than those of non-embryonic ones,the soluble sugar content kept steady,only had some rise during bud differentiation;α-amylase activity kept steady.The β-amylase activity in embryonic callus was all higher than that of non-embryonic callus,which indicated that β-amylase had a close correlation with differentiation process of cells.β-amylase showed bimodal curve in the whole culture process,the first peak appeared at the beginning stage of culture,and the second peak presented at bud differentiation phase.Conclusion] The study had important guiding significance in the research of high frequency regeneration of Xinjiang S.involucrata. |
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| Keywords: | Xinjiang Saussurea involucrata Callus Protein Soluble sugar Amylase |
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