Agarose gel electrophoresis of alkaline phosphatase isoenzymes in the serum of hyperthyroid cats |
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| Authors: | Horney Barbara S Farmer Andrea J Honor David J MacKenzie Allan Burton Shelley |
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| Institution: | Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Prince Edward Island, C1A 4P3, Canada. |
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| Abstract: | Cats with hyperthyroidism (increased serum thyroxine (T(4))] commonly have increased serum alkaline phosphatase (ALP) activity in addition to other serum biochemical abnormalities. Serum biochemical profiles were obtained from 10 hyperthyroid cats which had increased serum ALP. Agarose gel electrophoresis of serum from these cats was performed and stained for alkaline phosphatase activity. Alkaline phosphatase activity was calculated for each of the separate bands obtained, and the results were compared to those of tissue extracts, serum from normal cats, and serum from normothyroid cats with increased serum ALP activity. The hyperthyroid cats had increased ALP activity in bands corresponding to isoenzymes originating in the liver, bone, and an unidentified tissue source. |
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| Keywords: | feline hyperthyroidism alkaline phosphatase isoenzyme electrophoresis |
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