| 滇黄芩毛状根的诱导及其黄芩苷含量测定 |
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| 引用本文： | 步怀宇,岑举人,王英娟,赵宇玮,李娜,毕彦博.滇黄芩毛状根的诱导及其黄芩苷含量测定[J].广西农业生物科学,2010(1):179-184. |
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| 作者姓名： | 步怀宇 岑举人 王英娟 赵宇玮 李娜 毕彦博 |
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| 作者单位： | 西北大学西部资源生物与现代生物技术教育部重点实验室;陕西省生物技术重点实验室; |
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| 基金项目： | 陕西省教育厅基金(09JS080;09JK775);;西北大学研究生创新基金(09YSY36)共同资助 |
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| 摘 要： | 本文利用发根农杆菌A grobacterizum rhizogenes1.2556感染滇黄芩再生苗的茎段和叶片,建立了毛状根培养及其植株再生体系。毛状根可直接从受伤的茎、叶外植体表面产生,在无外源激素的MS固体和液体培养基上自主生长,表现出典型的发根特征。毛状根茎段的诱导率较叶片高,最高可达到14.44%;经rolB基因PCR分析和甘露碱纸电泳检测,证明Ri质粒T-DNA已整合到滇黄芩基因组中并表达;毛状根在附加6-BA2mg/L和NAA0.2mg/L的MS固体培养基上直接诱导不定芽,并在MS培养基上生根,形成再生植株。获得的毛状根系经MS液体培养基培养30d后通过HPLC都能检测到黄芩苷,其中1个转化系黄芩苷含量为2.59%,是药材黄芩的0.20倍,而从3年单位时间黄芩苷生成量计算,毛状根是药材黄芩的7.18倍。本研究建立的毛状根培养体系,将对滇黄芩转基因技术的完善和利用毛状根生产黄芩苷的生物转化提供了实验基础。
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| 关 键 词： | 滇黄芩 毛状根 黄芩苷 发根农杆菌 |
Agrobacterium rhizogenes Mediated Transformation of Scutellaria amoena and Production of Baicalin in Hairy Roots |
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| Bu Huaiyu Cen Juren Wang Yingjuan Zhao Yuwei Li Na Bi Yanbo Key Laboratory of Resource Biology , Biotechnology in Western China,Northwest University,Ministry of Education,Shaanxi Provincial Key Laboratory of Biotechnology,Xi'an.Agrobacterium rhizogenes Mediated Transformation of Scutellaria amoena and Production of Baicalin in Hairy Roots[J].Journal of Guangxi Agricultural and Biological Science,2010(1):179-184. |
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| Authors: | Bu Huaiyu Cen Juren Wang Yingjuan Zhao Yuwei Li Na Bi Yanbo Key Laboratory of Resource Biology Biotechnology in Western China Northwest University Ministry of Education Shaanxi Provincial Key Laboratory of Biotechnology Xi'an |
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| Institution: | Bu Huaiyu Cen Juren Wang Yingjuan Zhao Yuwei Li Na Bi Yanbo Key Laboratory of Resource Biology , Biotechnology in Western China,Northwest University,Ministry of Education,Shaanxi Provincial Key Laboratory of Biotechnology,Xi'an,710069 |
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| Abstract: | The objective of this research was to establish an efficient system of genetic transformation and plant regeneration from hairy roots by infecting the leaf sections and stem segments of in vitro Scutellaria amoena C. H.Wright plantlets. Hairy roots were induced from the wounded surface of the explants on hormone free MS medium after infection by Agrobacterium rhizogenes strain 1.2556 and which showed the representative characteristic of hairy root. The frequency of stem segments was higher than that of leaf... |
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| Keywords: | Scutellaria amoena C H Wright Hairy roots Baicalin Agrobacterium rhizogenes |
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