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| 隐丹参酮对人胆管癌HCCC-9810细胞增殖、survivin基因及caspase-3蛋白表达的影响 | |
| 引用本文： | 陈春雷,弓建华,缪辉来,林晖,赵芳良.隐丹参酮对人胆管癌HCCC-9810细胞增殖、survivin基因及caspase-3蛋白表达的影响[J].湛江医学院学报,2013,31(1):4-7. |
| 作者姓名： | 陈春雷 弓建华 缪辉来 林晖 赵芳良 |
| 作者单位： | 1. 广东省湛江市第二人民医院,广东湛江,524003 2. 广东医学院,广东湛江,524023 3. 广东医学院附属医院外科,广东湛江,524001 |
| 基金项目： | 广东省中医药局建设中医药强省立项资助（No.20111090） |
| 摘 要： | 目的 了解隐丹参酮对人胆管癌HCCC-9810细胞增殖及凋亡相关因子surv1vin、caspase-3表达的影响.方法 HCCC-9810细胞用不同浓度隐丹参酮处理24、48、72 h,分别用MTT法、RT-PCR、Western blot检测细胞生长及survivin mRNA、caspase-3蛋白表达.结果 隐丹参酮抑制HCCC-9810细胞增殖;随时间延长,HCCC-9810细胞中survivin mRNA表达逐渐降低,而caspase-3蛋白表达逐渐增高(P＜0.05).结论 隐丹参酮可通过下调survivin基因表达及上调caspase-3蛋白表达来诱导HCCC-9810细胞凋亡. |
| 关 键 词： | 隐丹参酮 胆管癌 survivin caspase-3 |
Effect of cryptotanshinone on proliferation and expression of survivin gene and caspase-3 protein in HCCC-9810 cells |
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| HEN Chun-lei , GONG Jian-hua , MIAO Hui-lai , LIN Hui , ZHAO Fang-liang.Effect of cryptotanshinone on proliferation and expression of survivin gene and caspase-3 protein in HCCC-9810 cells[J].Journal of Guangdong Medical College,2013,31(1):4-7. | |
| Authors: | HEN Chun-lei GONG Jian-hua MIAO Hui-lai LIN Hui ZHAO Fang-liang |
| Institution: | 1.Second People＇s Hospital of Zhanjiang,Zhanjiang 524003,China) |
| Abstract: | Objective To investigate the effect of cryptotanshinone on proliferation and expression of apoptosis-related factors including survivin and caspase-3 in HCCC-9810 cells.Methods After treated with different concentrations of cryptotanshinone for 24 h,48 h and 72 h,growth and expression of survivin mRNA and caspase-3 protein in HCCC-9810 cells were measured by MTT assay,RT-PCR and Western blot,respectively.Results The proliferation of HCCC-9810 cells was inhibited by cryptotanshinone.Survivin mRNA decreased but caspase-3 protein increased with treatment time in HCCC-9810 cells（P0.05）.Conclusion Cryptotanshinone can induce the apoptosis of HCCC-9810 cells via downregulation of survivin gene and upregulation of caspase-3 protein. |
| Keywords: | cryptotanshinone cholangiocarcinoma survivin caspase-3 |
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