Significance of NF-кB in immunopathogenesis of Graves disease |
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| Authors: | MIN Xun CHEN Dai-xiong WANG Yong-lun FANG Ning WAN Wei-hong LIU Jin-wei XIAO Yu ZHOU Zhen-zhong |
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| Institution: | 1.Laboratory of Clinical Medicine, Affiliated Hospital of Zunyi Medical College, 2Key Laboratory of Cell Engineering of Guizhou Province, Zunyi 563003, China.E-mail:cellgene@163.com |
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| Abstract: | AIM:To investigate the activity of NF-кB in peripheral blood mononuclear cells (PBMCs) from patients with Graves disease (GD) and the significance in immunopathogenesis of GD.METHODS:Peripheral blood was collected from 22 untreated GD, 20 treated GD with tapazole more than 1 year, and 25 healthy volunteers.PBMCs were isolated from the blood by histopaque-1077 density-gradient centrifugation.The activity of NF-кB in PBMCs was analyzed using gel electrophoretic mobility shift assay (EMSA).The contents of IL-1β, IL-6 and TNF-α were tested by radioimmunoassay.RESULTS:The activity of NF-кB in PBMCs of untreated GD group was increased remarkably, compared with that in the treated group and control (P<0.05).The contents of IL-1β, IL-6 and TNF-α in untreated group were significantly higher than those in treated GD and control group (P<0.05).A positive correlation between NF-кB activity and IL-6 level in untreated GD group and treated GD group was observed.CONCLUSION:The activity of NF-кB in PBMCs with GD patients is increased significantly, which might play an important role in the immunopathogenesis of GD. |
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