Effect of trastuzumab (herceptin) on the apoptosis induced by ZD1839 (iressa) in lung cancer A549 cells |
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| Authors: | LI Wen-yu GUO Ai-lin YANG Su-qing CHEN Zhi-hong XIE Zhi WU Yi-long |
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| Institution: | Cancer Center, Research Center of Medicine, Guangdong Provincial People′s Hospital, Guangzhou 510080, China.E-mail:gzyilong@hotmail.com |
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| Abstract: | AIM: ZD1839 and trastuzumab are reported to improve the therapeutic efficacy of treatment for non-smallcell lung cancer (NSCLC) and breast cancer, respectively, although the effectiveness of either drug alone is not satisfactory. NSCLC cells often express both EGFR and HER2. We therefore investigated whether a combination of ZD1839 and trastuzumab had an additive or synergistic antitumor effect. METHODS: MTT was used to measure the inhibitory effects of ZD1839 (iressa) and trastuzumab (herceptin) on the growth of A549 cells. The cell apoptosis was studied by DAPI staining, and Annexin V/PI double labeling. RESULTS: The inhibitory action of cell growth was seen in A549 cells dealing with ZD1839 and trastuzumab. They inhibited the growth of the human lung cancer cell line A549 in a concentration and time dependent manners. Compared with either ZD1839 or trastuzumab alone, combination with curcumin respectively increased the growth inhibition rate and increased apoptosis of A549 cells (P<0.05) significantly, suggesting the synergistic actions of the two drugs. CONCLUSION: The results suggest that combination treatment with ZD1839 and trastuzumab might have improved therapeutic efficacy against NSCLC cells expressing both EGFR and HER2. |
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| Keywords: | Iressa Herceptin Lung neoplasms A549 cells |
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