| 广西桑树种质资源亲缘关系的SRAP分析 |
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| 引用本文： | 林强,朱方容,邱长玉,陈小青,赵卫国,李乙,曾燕蓉,朱光书,唐燕梅,宾荣佩.广西桑树种质资源亲缘关系的SRAP分析[J].广西农业科学,2011,42(4):358-363. |
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| 作者姓名： | 林强 朱方容 邱长玉 陈小青 赵卫国 李乙 曾燕蓉 朱光书 唐燕梅 宾荣佩 |
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| 作者单位： | 1. 广西蚕业科学研究院,南宁,530007
2. 中国农业科学院蚕业研究所,江苏镇江,212018 |
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| 基金项目： | 现代农业产业技术体系建设专项项目，广西科学研究与技术开发计划项目，广西自然科学基金 |
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| 摘 要： | 【目的】从DNA分子水平了解广西地方桑树种质资源的系统发育和亲缘关系。【方法】利用SRAP分子标记技术对28份广西地方桑树种质资源进行SRAP多态性和聚类分析。【结果】筛选的22对SRAP引物组合从28份材料中共扩增出144条谱带,其中45条为多态性带,多态性带比率为31.81%。每对引物组合的谱带数和多态性带数分别为6.55条和2.05条。供试材料间的遗传相似系数为0.8264～1.0000,平均为0.8944。UPGMA聚类结果表明,28份桑树材料可分成3类,Ⅰ类包括20份广西地方种质资源,Ⅱ类包括7份广西选育的种质资源,Ⅲ类包括1份广西种质资源。【结论】广西桑树种质资源品系间存在较高的遗传变异,桑树地方种质资源的遗传多样性和亲缘关系与地域性有密切关系,可为桑树种质资源的分类、保护和育种利用提供参考。
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| 关 键 词： | 桑树 种质资源 遗传多样性 SRAP 聚类分析 |
Assessing the phylogenetic relationship amongst Guangxi mulberry (Morus atropurpurea Roxb.) germplasm resources using SRAP markers |
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| LIN Qiang,ZHU Fang-rong,QIU Chang-yu,CHEN Xiao-qing,ZHAO Wei-guo,LI Yi,ZENG Yan-rong,ZHU Guang-shu,TANG Yan-mei,BIN Rong-pei.Assessing the phylogenetic relationship amongst Guangxi mulberry (Morus atropurpurea Roxb.) germplasm resources using SRAP markers[J].Guangxi Agricultural Sciences,2011,42(4):358-363. |
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| Authors: | LIN Qiang ZHU Fang-rong QIU Chang-yu CHEN Xiao-qing ZHAO Wei-guo LI Yi ZENG Yan-rong ZHU Guang-shu TANG Yan-mei BIN Rong-pei |
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| Institution: | 1 Guangxi Academy of Sericulture Sciences,Nanning 530007,China;2 Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang,Jiangsu 212018,China) |
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| Abstract: | Objective]The present experiment was conducted to study the phylogenetic relationship amongst Guangxi mulberry（Morus atropurpurea Roxb.）germplasm resources using SRAP（sequence-related amplified polymorphism） markers.Method]The polymorphism amongst 28 Guangxi local mulberry germplasm resources,belonging to Guangdong mulberry family,was assessed using SRAP molecular marker technique and their genetic diversity was analyzed using cluster analysis.Result]The results showed the amplification of 144 bands in 28 mulberry germplasm using 22 pairs of SRAP primer combinations.Of which,45 bands（31.81%） showed polymorphism.On an average,the number of total and polymorphic bands/primer pair primer combination was recorded as 6.55 and 2.05,respectively.The genetic similarity coefficients among mulberry resources ranged from 0.8264 to 1.0000,with an average of 0.8944.UPGMA results showed 3 clear clusters for the 28 mulberry materials in dendrogram.The first group included 20 local mulberry materials,the second included 7 self-breeding mulberry materials and the third had only one material.Conclusion]High genetic variations amongst Guangxi mulberry germplasm resources were observed,and the genetic diversity and phylogenetic relationship of local mulberry germplasm were found in accordance with their habitat.The results of our experiment will be helpful in classification and breeding of the mulberry genotypes. |
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| Keywords: | mulberry（Morus atropurpurea Roxb） genetic diversity SRAP clustering analysis |
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