| 陕西省小麦白粉菌群体毒性结构及遗传多样性 |
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| 引用本文： | 李莹,马康杰,宫丹丹,吴蕾,李强,王保通.陕西省小麦白粉菌群体毒性结构及遗传多样性[J].植物病理学报,2022,52(3):396-403. |
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| 作者姓名： | 李莹 马康杰 宫丹丹 吴蕾 李强 王保通 |
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| 作者单位： | 西北农林科技大学植物保护学院,旱区作物逆境生物学国家重点实验室,杨凌 712100 |
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| 基金项目： | 国家重点研发计划（2016YFD0300705、2018YFD0200404、2018YFD0200501）; |
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| 摘 要： | 为明确陕西省小麦白粉菌群体的毒性频率和遗传多样性,利用34个含有已知抗白粉病基因的小麦品种(系)和5对多态性ISSR分子标记,分别对2016年渭南、西安、咸阳、宝鸡、汉中和安康等6市的15个乡镇160个小麦白粉菌单孢子堆菌株进行毒性频率分析。结果显示:供试小麦白粉菌群体对Pm1、Pm2、Pm3b、Pm3c、Pm3e、Pm3f、Pm6、Pm7、Pm8、Pm19和Pm1+2+19的毒性频率在60%~100%之间,表明这些抗性基因已丧失抗性,在生产上已经丧失利用价值,对Pm4b、Pm24、Pm2+6、Pm2+Mld、Pm2+6+?、Pm4b+Mli、Pm"Era"、Pm"XBD"和Pm21的毒性频率低于20%,表明这些抗性基因抗性良好,可在生产中利用。选取其中93个单孢子堆菌株进行遗传多样性分析,结果表明白粉菌地理群体间遗传距离在0.020 4~0.103 7之间,其中宝鸡和渭南群体的遗传距离最近,汉中和咸阳群体的遗传距离最远。群体间遗传变异占总体变异的12.82%,群体内遗传变异占87.12%,表明遗传变异主要来自于群体内。Mantel Test分析表明,小麦白粉菌群体间遗传距离与地理距离相关性不大。
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| 关 键 词： | 陕西省 小麦白粉菌 毒性频率 遗传多样性 |
| 收稿时间： | 2021-03-04 |
Virulence and genetic diversity of Blumeria graminis f. sp.tritici population in Shaanxi Province |
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| LI Ying,MA Kangjie,GONG Dandan,WU Lei,LI Qiang,WANG Baotong.Virulence and genetic diversity of Blumeria graminis f. sp.tritici population in Shaanxi Province[J].Acta Phytopathologica Sinica,2022,52(3):396-403. |
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| Authors: | LI Ying MA Kangjie GONG Dandan WU Lei LI Qiang WANG Baotong |
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| Institution: | College of Plant Protection Northwest A＆F university, State Key Laboratory for Crop Stress Biology in Arid Areas, Yangling 712100, China |
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| Abstract: | To identify the virulence frequency and genetic diversity of Blumeria graminis f. sp. Tritici population, 34 wheat Cultivars (lines) with known Powdery mildew resistance genes and 5 pairs of ISSR markers were used in the study of virulence frequency and genetic diversity of isolates in Shaanxi Province respectively. The genetic diversity of 160 isolates from 15 towns in 6 cities, including Weinan, Xi′an, Xianyang, Baoji, Hanzhong and Ankang, Shaanxi Province, were collected in 2016. Results showed that the virulence frequencies of the resistance genes Pm1, Pm2, Pm3b, Pm3c, Pm3e, Pm3f, Pm6, Pm7, Pm8, Pm19 and Pm1+2+19 were 60%-100%, which meaned the Pm genes have lost resistance and value in production. While the virulence frequencies of Pm4b, Pm24, Pm2+6, Pm2+Mld, Pm2+6+?, Pm4b+Mli, Pm"Era", Pm"XBD" and Pm21 were less than 20%, which indicated the Pm genes have good resistance and value in wheat breeding. The analysis of 93 strains shows that the genetic distances were between 0.020 4-0.103 7, and the lowest value was between Baoji and Weinan, while the highest value was between Hanzhong and Xianyang. The genetic variation between populations accounted for 12.82% of the total variation, and the genetic variation within the population accounted for 87.12%, indicating that the genetic variation mainly come from within the population. Mantel Test illustrated that the genetic distance of wheat powdery mildew had little correlation with geographic distance. |
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| Keywords: | Shaanxi Province Blumeria graminis f sp tritici virulent frequency genetic diversity |
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