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| 用于遗传转化的花生植株再生体系研究 | |
| 引用本文： | 蒋菁,何新民,王兴军,唐洲萍,唐荣华.用于遗传转化的花生植株再生体系研究[J].广西农业科学,2009,40(12):1513-1518. |
| 作者姓名： | 蒋菁 何新民 王兴军 唐洲萍 唐荣华 |
| 作者单位： | 1. 广西农科院经济作物研究所,南宁,530007;广西作物遗传改良生物技术重点开放实验室,南宁,530007 2. 广西农科院经济作物研究所,南宁,530007 3. 山东农科院高新技术研究中心,济南,250100 |
| 摘 要： | 以广西花生主栽品种桂花17和桂花22的成熟种子胚小叶为外植体,探讨外植体处理方式、不同激素组合和蔗糖浓度对外植体不定芽诱导、继代、生根培养的影响。结果表明,在MS培养基中预培养0、4d的花生种子的胚小叶利于不定芽的诱导,具有较高的分化率;近叶柄1/3处横切的小叶不定芽分化率较远离叶柄部位的高。适宜小叶不定芽分化的蔗糖浓度为40～50g/L,40、50g/L蔗糖分别利于桂花22、桂花17不定芽的分化。培养基MS＋4.0mg/L 6-BA＋1.0mg/L NAA＋2.0mg/L AgNO3对桂花17不定芽的分化效果较佳,MS＋6.0mg/L 6-BA＋1.0mg/L NAA＋2.0mg/L AgNO3利于桂花22不定芽的分化,两者的不定芽分化率均达100%;继代培养基MS＋1.5mg/L 6-BA＋0.4mg/L NAA有利于促进两个花生品种的不定芽成苗,而最佳生根培养基为1/2 MS＋0.5mg/L 6-BA＋2.0mg/L NAA。已构建的花生植株再生体系,不定芽分化率高,再生植株多,适合进行花生的遗传转化。 |
| 关 键 词： | 花生 胚小叶 不定芽 分化 继代 生根 植株再生 |
In vitro plant regeneration system of peanut for genetic transformation |
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| JIANG Jing,HE Xin-min,WANG Xing-jun,TANG Zhou-ping,TANG Rong-hua.In vitro plant regeneration system of peanut for genetic transformation[J].Guangxi Agricultural Sciences,2009,40(12):1513-1518. | |
| Authors: | JIANG Jing HE Xin-min WANG Xing-jun TANG Zhou-ping TANG Rong-hua |
| Institution: | JIANG Jing1,3,HE Xin-min1,WANG Xing-jun2,TANG Zhou-ping1,TANG Rong-hua1(1 Cash Crops Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China,2 High-Tech Research Center,Shandong Academy of Agricultural Sciences,Jinan 250100,3 Guangxi Crop Genetic Improvement and Biotechnology Laboratory,China) |
| Abstract: | The present experiment was conducted to study the effects of different explant treatment methods,hormone combinations and sugar concentrations on the induction,subculture and rooting in adventitious bud derived from embryonic leaf of peanut and to develop a regeneration system for highly efficient genetic transformation studies in peanuts.The main cultivated varieties of Guangxi,viz.,Guihua 17 and Guihua 22 were used and their seeds were precultured for 0,4,8 and 12 days on MS medium.The results showed that... |
| Keywords: | peanut embryonic leaves adventitious bud differentiation subculture rooting plant regeneration |
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