| 荔枝、龙眼属间远缘杂种的获得及分子鉴定 |
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| 引用本文： | 赵玉辉,胡又厘,郭印山,周佳,傅嘉欣,刘成明,朱江,张明君.荔枝、龙眼属间远缘杂种的获得及分子鉴定[J].果树学报,2008,25(6):950-952,F0003. |
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| 作者姓名： | 赵玉辉 胡又厘 郭印山 周佳 傅嘉欣 刘成明 朱江 张明君 |
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| 作者单位： | 华南农业大学同艺学院,广州510642 |
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| 基金项目： | 围家自然科学基金，中国博士后科学基金，广东省科技计划项目，华南农业大学校长基金 |
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| 摘 要： | 以荔枝品种紫娘喜为父本,龙眼品种石硖、早熟龙眼、古山二号为母本,以及以紫娘喜为母本,石硖龙眼为父本,进行了远缘杂交研究。通过混合授粉和多组合杂交的方式,改善了属间杂交组合的不亲和性。结果表明,在紫娘喜×石硖、石硖×紫娘喜、早熟龙眼×紫娘喜以及古山二号×紫娘喜4个杂交组合中共获得杂交苗35株,经SRAP-PCR分析,在紫娘喜×石硖组合中鉴定出2株真杂种苗,杂种苗生长健壮,叶片宽大。研究的形态学和分子标记分析均显示获得了荔枝和龙眼的属间远缘杂种。
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| 关 键 词： | 荔枝 龙眼 远缘杂交 分子鉴定 |
Inter-generic hybrids obtained from cross between litchi and longan cul-tivars and their molecular identification |
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| ZHAO Yu-hui,HU You-li,GUO Yin-shan,ZHOU Jia,FU Jia-xin,LIU Cheng-ming,ZHU Jiang,ZHANG Ming-jun.Inter-generic hybrids obtained from cross between litchi and longan cul-tivars and their molecular identification[J].Journal of Fruit Science,2008,25(6):950-952,F0003. |
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| Authors: | ZHAO Yu-hui HU You-li GUO Yin-shan ZHOU Jia FU Jia-xin LIU Cheng-ming ZHU Jiang ZHANG Ming-jun |
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| Institution: | ZHAO Yu-hui,HU You-li,GUO Yin-shan,ZHOU Jia,FU Jia-xin,LIU Cheng-ming,ZHU Jiang,ZHANG Ming-jun(College of Horticulture,South China Agricultural University,Guangzhou,Guangdong 510642 China) |
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| Abstract: | Distant hybridization between litchi and longan cultivars was made by using mixed pollens for conquering the cross barrier between genera.Shixia,Zaoshulongyan and Gushan 2 longan cultivars were used as female crossed by lichi cultivar Ziniangxi,and Ziniangxi was used as female crossed by Shixia longan cultivar.Total of 35 seedlings were obtained from the 4 crossed combinations,including Ziniangxi × Shixia,Shixia × Ziniangxi,Zaoshulongyan × Ziniangxi and Gushan-2 × Zini-angxi.Identification of hybrid seedlings by SRAP-PCR showed two seedlings were real hybrid of Ziniangxi × Shixia.They are stalwart and have spacious leaf blade,indicating the real distant hybrid of litchi and longan. |
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| Keywords: | Litchi Longan Distant hybridization Molecular identification |
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