| 转逆境诱导型启动子SWPA2驱动Cu/Zn SOD和APX基因甘薯(Ipomoea batatas(L).)Lam.)耐盐性 |
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| 引用本文： | 王欣,过晓明,李强,唐忠厚,郭尚洙,马代夫.转逆境诱导型启动子SWPA2驱动Cu/Zn SOD和APX基因甘薯(Ipomoea batatas(L).)Lam.)耐盐性[J].分子植物育种,2011,9(6):754-759. |
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| 作者姓名： | 王欣 过晓明 李强 唐忠厚 郭尚洙 马代夫 |
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| 作者单位： | 1. 中国农业科学院甘薯研究所/江苏徐州甘薯研究中心,徐州,221121
2. 环境生物工程研究中心,韩国生命工学研究院,大田,305-806 |
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| 基金项目： | 国家863计划，国家科技支撑计划 |
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| 摘 要： | 本研究以盐胁迫下逆境诱导型启动子SWPA2驱动转Cu/Zn SOD和APX基因甘薯叶片一些生理指标的变化研究转基因甘薯耐盐性.结果表明:无胁迫时,转基因甘薯叶的生理指标与未转基因植株无明显差异.相同胁迫条件下,转基因甘薯叶的超氧化物歧化酶(SOD),抗坏血酸过氧化物酶(APX),过氧化物酶(POD),过氧化氢酶(CAT...
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| 关 键 词： | 逆境诱导型启动子SWPA2 Cu/ZnSOD-APX基因 转基因甘薯 耐盐性 |
Salt Tolerance of Transgenic Sweetpotato Expressing the Gene Both CuZn Superoxide Dismutase and Ascorbate Peroxidase with the Stress-inducible SWPA2 Promoter |
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| Wang Kin,Guo Xiaoming,Li Qiang,Tang Zhonghou,KWAK Sangsoo,Ma Daifu.Salt Tolerance of Transgenic Sweetpotato Expressing the Gene Both CuZn Superoxide Dismutase and Ascorbate Peroxidase with the Stress-inducible SWPA2 Promoter[J].Molecular Plant Breeding,2011,9(6):754-759. |
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| Authors: | Wang Kin Guo Xiaoming Li Qiang Tang Zhonghou KWAK Sangsoo Ma Daifu |
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| Institution: | 1 Sweetpotato Research Institute, Chinese Academy of Agricultural Sciences/Xuzhou Sweetpotato Research Center, XuZhou, 221121; 2 Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806 |
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| Abstract: | Some physiological indexes in transgenic sweetpotato (Ipomoea batatas L. cv. Yulmi) plants expressing both CuZn superoxide dismutase (CuZnSOD) and ascorbate peroxidase (APX) genes with the stress-inducible SWPA2 promoter were evaluated under different concentrations of NaCl treatment. The result indicated, physiological indexes were no remarkable differences without NACl stress between the TS and the NS. The levels of superoxide dismutase (SOD), ascorbate peroxidease (APX), peroxidase (POD) and catalase (CAT) activity in leaves of transgenic sweetpotato (TS) were always higher than non-transformed sweet potato (NS) under the same NaCl stress, and we observed remarkable differences under 100 mmol/L NaCl concentration. The root of TS was longer than the NS, and the decline range of chlorophyll and malonaldehyde (MDA) content in leaves of TS was below the NS. These results indicate that sweet potato over-expressing CuZnSOD-APX gene might efficiently tolerate the salt stress. |
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| Keywords: | The stress-inducible SWPA2 promoter CuZnSOD-APX gene Transgenic sweet potato Salt tolerance |
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