| 黄瓜作图亲本间分子标记的多态性分析 |
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| 引用本文： | 黄焕焕,张桂华,韩毅科,魏爱民,杜胜利,张显.黄瓜作图亲本间分子标记的多态性分析[J].华北农学报,2007,22(2):47-49. |
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| 作者姓名： | 黄焕焕 张桂华 韩毅科 魏爱民 杜胜利 张显 |
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| 作者单位： | 1. 西北农林科技大学,园艺学院,陕西,杨凌,712100
2. 天津科润黄瓜研究所,天津300192 |
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| 基金项目： | 天津市农业科技成果转化与推广资助项目(0502070)，国家“863”引导项目(2005AA001030) |
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| 摘 要： | 利用AFLP,SRAP和SSR3种不同类型的分子标记技术,研究了作图亲本F-3与HZL04-1间的多态性。结果表明,这3种标记技术多态性检测效率不同,其中,AFLP揭示作图亲本间多态性效率最高,SRAP次之,SSR多态性检测效率最低。选择高效揭示多态性的分子标记技术是提高黄瓜遗传图谱构建效率的关键。分析探讨了几种分子标记技术的优缺点,认为应用AFLP,SRAP构建黄瓜遗传图谱为最佳,SSR可以作为补充的标记。
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| 关 键 词： | 黄瓜 遗传图谱 AFLP SRAP SSR |
| 文章编号： | 1000-7091（2007）02-0047-03 |
| 修稿时间： | 2006-09-26 |
Molecular Marker Polymorphism Between Mapping Parents in Cucumis sativus L. |
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| HUANG Huan-huan,ZHANG Gui-hua,HAN Yi-ke,WEI Ai-min,DU Sheng-li,ZHANG Xian.Molecular Marker Polymorphism Between Mapping Parents in Cucumis sativus L.[J].Acta Agriculturae Boreali-Sinica,2007,22(2):47-49. |
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| Authors: | HUANG Huan-huan ZHANG Gui-hua HAN Yi-ke WEI Ai-min DU Sheng-li ZHANG Xian |
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| Institution: | 1. College of Horticulture, Northwest Science and Technology University of Agriculture and Forestry, Yangling 712100, China;2.Tianjin Kernel Cucumber Research Institute,Tianjin 300192, China |
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| Abstract: | Polymorphism of between mapping parents,F-3 and HZL04-1,in C.sativus L. were analyzed by three kinds of molecular marker technology(AFLP,SRAP and SSR).The results showed that AFLP was the most efficient,and SSR the least.This indicated that the key for mapping in C.sativus L.was to select high efficient molecular marker technology.The advantages and disadvantages of some kinds of molecular markers were also discussed.In this study,AFLP,SRAP are better for reference linkage map construction in Cucumis sativus L.,and SSR is only a kind of complementary molecular marker. |
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| Keywords: | Cucumis sativus L Genetic map AFLP SRAP SSR |
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