| 不同浓度NO3- 胁迫下黄瓜幼苗根系分生区细胞内Ca2+分布变化的差异 |
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| 引用本文： | 杨凤娟,魏珉,苏秀荣,王秀峰.不同浓度NO3- 胁迫下黄瓜幼苗根系分生区细胞内Ca2+分布变化的差异[J].园艺学报,2009,36(9):1291-1298. |
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| 作者姓名： | 杨凤娟 魏珉 苏秀荣 王秀峰 |
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| 作者单位： | ( 山东农业大学, 作物生物学国家重点实验室, 山东泰安271018; 山东农业大学园艺科学与工程学院, 山东泰安 271018; 山东农业大学化学与材料科学学院, 山东泰安271018) |
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| 基金项目： | 国家自然科学基金，山东农业大学中青年创新基金 |
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| 摘 要： | 以‘新泰密刺’黄瓜为试材, 采用焦锑酸钙沉淀的电镜细胞化学方法, 研究不同浓度NO3- 胁迫下黄瓜幼苗根系分生区细胞内Ca2+的分布变化, 以期为探讨不同浓度NO3- 胁迫下细胞Ca2+行为特征与黄瓜对NO3- 适应性之间的关系。结果表明, 对照生长条件下(14 mmol·L-1 NO3- ) , 黄瓜根系分生区细胞内Ca2+主要出现在细胞膜和小液泡内, 线粒体、细胞质和细胞核及核仁内也有少量的较小颗粒钙沉淀。而其它浓度NO3- 胁迫下, 其根系分生区细胞Ca2+定位分布变化明显。在56和140 mmol·L -1NO3- 浓度下, Ca2+有向细胞基质分布的趋势, 其细胞间隙、细胞质和线粒体中亦出现大量钙沉淀颗粒。且不同处理浓度下, 线粒体和小液泡数量及结构变化较大。而当NO3- 浓度达182 mmol·L -1时, 根系分生区各细胞器内钙沉淀颗粒明显减少, 此时Ca2+的变化是各细胞器及膜系统遭严重破坏的结果。综上可知, 黄瓜幼苗可通过根系分生区液泡内Ca2+向细胞基质分布变化, 来增加对高浓度NO3- 胁迫的抗性。
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| 关 键 词： | 黄瓜 NO3- 浓度 焦锑酸钙沉淀 细胞生物化学 钙离子 |
| 收稿时间： | 2009-3-31 |
| 修稿时间： | 2009-6-26 |
The Differences of Subcellular Ca2+Distribution in Root Meristem Region of Cucumber Seedling Under Different NO3- Concentrations Stress |
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| YANG Feng-juan,WEI Min,SU Xiu-rong,WANG Xiu-feng.The Differences of Subcellular Ca2+Distribution in Root Meristem Region of Cucumber Seedling Under Different NO3- Concentrations Stress[J].Acta Horticulturae Sinica,2009,36(9):1291-1298. |
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| Authors: | YANG Feng-juan WEI Min SU Xiu-rong WANG Xiu-feng |
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| Institution: | ( State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong 271018, China; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, China; College of Chem istry and Material Science, Shandong Agricultural University, Tai'an,Shandong 271018, China) |
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| Abstract: | The changes of calcium level in rootmeristem region of cucumber seedling (‘XintaiMici’) under different NO3- concentrations stress were determined by the imp roved cytochemical method of calcium pyroantimonate p recip itation. After 7 days of treatment, under op timum NO3- (14 mmol·L -1) conditions,calcium antimonite deposits, which indicates calcium distribution, were mainly localized in cellmembrane and vacuoles, as a storeroom of Ca2+, and smaller amounts of calcium p recip itates randomly resided in mitochon2dria, cytop lasm and nucleus. But under excessive NO3-stress, the distribution of Ca2+ in subcellular was changed greatly. After 7 days treated with 56 and 140 mmol·L-1 NO3-, Ca2+level in the intercellular spaces, cytop lasm and mitochondria elevated. With the increasing NO3-concentrations, the shape and amount of mitochondria and vacuoleswere changed greatly. Higher NO3-concentrations (182 mmol·L-1) caused a series of disorders of metabolic p rocesses and serious damages of membrane system of organelles in cucumber seedling roots, thus, Ca2+deposits in each compartments became smaller significantly. The result indicated that the change of Ca2+ from vacuoles to hyalop lasm can reduce the NO3-stress in cucumber seedlings. |
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| Keywords: | cucumber seedling 3 calcium pyroantimonate precipitation cytochemistry 2+ |
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