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| 多枝赖草谷胱甘肽还原酶基因的克隆及分析 | |
| 引用本文： | 史仁玖,赵茂林,杨 清.多枝赖草谷胱甘肽还原酶基因的克隆及分析[J].西北农林科技大学学报(社会科学版),2006,34(2):61-67. |
| 作者姓名： | 史仁玖 赵茂林 杨 清 |
| 作者单位： | 1. 泰山医学院,生物化学与分子生物学教研室,山东,泰安,271000 2. 北京农业生物技术研究中心,北京,100089 3. 南京农业大学,生命科学学院,江苏,南京,210095 |
| 基金项目： | 中国科学院资助项目;北京市自然科学基金 |
| 摘 要： | 以盐胁迫处理的多枝赖草(Leymus multicaulis)植株新鲜叶片为材料,根据其他植物谷胱甘肽还原酶(GR)氨基酸保守区序列设计简并引物,通过RT—PCR扩增到1个408 bp的cDNA片段(Genbank注册号为 AY781786);利用DNAMAN软件将5’和3’RACE获得的5’和3’端序列,拼接成1个全长1 580 bp的cDNA序列,其包含1个1 140 bp的开放阅读框架,该阅读框架编码380个氨基酸;多枝赖草谷胱甘肽还原酶氨基酸序列与其他植物的同源性为77％～92％,定量PCR结果表明,GR基因的表达随着盐胁迫时间和盐浓度的增加而加强。 |
| 关 键 词： | 多枝赖草 谷胱甘肽还原酶 盐胁迫 GR基因的表达 |
| 文章编号： | 1671-9387(2006)02-0061-07 |
| 收稿时间： | 2005-06-13 |
| 修稿时间： | 2005年6月13日 |
Cloning and analysis of glutathione reductase gene from Leymus multicaulis |
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| SHI Ren-jiu,ZHAO Mao-lin,YANG Qing.Cloning and analysis of glutathione reductase gene from Leymus multicaulis[J].Journal of Northwest Sci-Tech Univ of Agr and,2006,34(2):61-67. | |
| Authors: | SHI Ren-jiu ZHAO Mao-lin YANG Qing |
| Institution: | 1.Department of Biochemistry, Taishan Medical College, Taian ,Shandong 271000 ,China ; 2.Beijing Agro-biotechnology Research Center, Beifing 100089,China;3.College of Life Science, Nanjing Agricultural University, Nanjing ,Jiangsu 210095, China |
| Abstract: | According to amino acid sequence from glutathione reductase in other plants,a pair of degenerate primers were designed and a 408 bp(Genebank Accession number:AY781786)cDNA fragment was obtained by RT-PCR from Leymus multicaulis under salt stress, 5' cDNA end and 3' cDNA end was obtained by RACE. The total cDNA of gene was 1 580 bp in length,including an open reading,encoding 380 amino acids. Its sequences shared high similarity at polypeptide level with the sequences from the plants of Oryza saliva , Zea mays, Vitis vinifera , Zinnia elegans, Nicotiana tabacum , Soybean and Glycine max. RT-PCR analysis indicated that the gene was up-regulated under salt stresses. |
| Keywords: | Leymus multicaulis glutathione reductase salt stress GR gene expression |
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