Mouse early integral embryo induces expression of endometrial integrin β3 and leukaemia-inhibitory factor,and improves uterine receptivity in mice |
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| Authors: | LI Ting-ting FANG Cong JIA Lei YUE Chao-min |
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| Institution: | Center for Reproductive Medicine, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou 510655, China |
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| Abstract: | AIM: To investigate the changes of endometrial receptivity under the effect of mouse embryo both in vitro and in vivo, and to figure out which part of the embryo induces the change. METHODS: Scanning electron microscope was applied to observe the pinpode formation on day 4 endometrium both in vitro and in vivo. The expression of integrin β3 and leukaemia-inhibitory factor(LIF) on day 2 pregnant mouse endometrium , day 4 endometrium after co-culture for 2 d with day 2 embryo , blastomere and zona pellucida as well as control group in vitro were detected by the methods of fluorescent quantitative PCR, immunohistochemistry and Western blotting. The same tests were conducted in the in vivo part of the experiment with the integral embryo or different parts of the embryo being transferred to pseudopregnant mouse uterus. On day 4 of the pregnancy, the endometrium was extracted to carry out the tests. RESULTS: After co-cultured for 2 d with whole embryo, the experssion of integrin β3 and LIF was higher than that in any other group in the in vitro part. The expression of integrin β3 and LIF on day 4 of normal pregnancy was higher than that in any other group in the in vivo experiments. CONCLUSION: Mouse embryo as a whole is able to induce better endometrial receptivity, while any separated part of embryo, such as blastomere or zona pellucida, couldn't. |
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| Keywords: | Embryo Integrin β3 Leukaemia-inhibitory factor Endometrial receptivity |
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