Inflammatory cytokines up-regulate FAT/CD36 expression in renal cells loaded by fatty acids |
| |
| Authors: | WAN Ke-qiang LIAO Jun-lei ZHAO Lei LI Qing CHEN Ya-xi RUAN Xiong-zhong |
| |
| Institution: | Chongqing Key Laboratory of Lipid and Glucose Metabolism,Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing 400016, China |
| |
| Abstract: | AIM: To investigate the effects of inflammatory cytokines on the expression of fatty acid transporter (FAT/CD36) in renal cells loaded by fatty acids. METHODS: Human mesangial cells (HMCs) and renal tubular epithelial HK-2 cells were treated with palmitate at concentrations of 0 mmol/L, 0.02 mmol/L, 0.04 mmol/L, 0.08 mmol/L, 0.16 mmol/L and 0.32 mmol/L for 24 h. The expression of FAT/CD36 at mRNA and protein levels was detected by real-time PCR and Western blotting,respectively. The renal cells were treated with palmitate at concentration of 0.04 mmol/L combined with TNF-α (25 μg/L) or IL-6 (20 μg/L) for 24 h. The effect of inflammatory cytokines on the mRNA and protein levels of FAT/CD36 in the renal cells was also investigated. Oil red O staining was used to determine the intracellular lipid droplet formation. The intracellular triglyceride (TG) and free fatty acid (FFA) were measured by enzymic assay and ELISA, respectively. RESULTS: Palmitate loading dose-dependently increased the expression of FAT/CD36 at mRNA and protein levels in both HMCs and HK-2 cells. The inflammatory cytokines further increased the expression of FAT/CD36 at mRNA and protein levels in both cells loaded by palmitate. Oil red O staining, TG detection and FFA assay showed that the inflammatory cytokines increased intracellular lipid levels in both HMCs and HK-2 cells. CONCLUSION: Inflammatory cytokines up-regulate the expression of FAT/CD36 in renal cells loaded by fatty acids and exacerbate the intracellular lipid accumulation. |
| |
| Keywords: | Mesangial cells HK-2 cells Inflammation Palmitates CD36 fatty acid transporter |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|
