Lovastatin-induced G1 phase synchronization in human endometrial cancer JEC cells |
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| Authors: | SHEN Hui-min LI Xiao-mao YANG Yue-bo WANG Xiao-yun |
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| Institution: | Department of Obstetrics and Gynecology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China |
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| Abstract: | AIM: To investigate the method of inducing G1 phase synchronization in human endometrial cancer JEC Cells by lovastatin and the cell cycle progress of JEC cells after desynchronization. METHODS: The doubling time of JEC cells was detected by Cell Counting Kit-8 (CCK-8) assay. To determine the best lovastatin concentrations for G1 synchronization, JEC cells were treated with lovastatin at concentrations of 10, 20, 30 and 40 μmol/L for 1× doubling time, and the cell cycle was detected using flow cytometry (FCM). To determine the best period of lovastatin treatment to achieve G1 synchronization, JEC cells were treated with lovastatin at the best concentration for 0.5× to 2× doubling time, and the cell cycle was detected every 4 h using FCM. Furthermore, the cell cycle progress of JEC cells after desynchronization was also observed. RESULTS: The doubling time of JEC cells was almost 24 h. Treatment with lovastatin at the concentration of 20 μmol/L for 24 h achieved maximum G1 arrest in JEC cells. Minimum G1 phase and maximum S phase were observed after desynchronization for 16 h. CONCLUSION: Maximum G1 synchronization of JEC cells is induced by lovastatin at the concentration of 20 μmol/L for 24 h. The JEC cells show minimum G1 phase and maximum S phase after desynchronization for 16 h. |
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| Keywords: | Lovastatin JEC cells Cell cycle G1 phase synchronization |
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