Role of apolipoprotein E in cholesterol efflux mediated by ABCA1 and ABCG1 |
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| Authors: | LU Yan-zhen ZHANG Cui-ying ZHANG Li-li SONG Juan JIA Jian-tao |
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| Institution: | 1. Department of Pathophysiology, Changzhi Medical College, Changzhi 046000, China;2. Department of Physiology, Changzhi Medical College, Changzhi 046000, China |
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| Abstract: | AIM:To investigate the role of apolipoprotein E(ApoE) in cholesterol efflux mediated by ATP-binding cassette transporter A1(ABCA1) and ATP-binding cassette transporter G1(ABCG1). METHODS:RAW 264.7 cells were seeded in either 6-well or 24-well plates, and then incubated with 20 mg/L low-density lipoprotein receptor gene knockout(LDLr-/-) mouse lipoprotein 20 mg/L ApoE gene knockout(ApoE-/-) mouse lipoprotein or culture medium alone. The changes of intracellular lipid content were measured by transmission electron microscopy and enzymatic colorimetric method. The cholesterol efflux was determined by liquid scintillation. The mRNA and protein levels of ABCA1 and ABCG1 were detected by real-time PCR and Western blotting, respectively. RESULTS:The ApoE-/- mouse lipoprotein increased the content of intracellular cholesterol ester by 60% compared with the control cells. In addition, ApoE-/- mouse lipoprotein treatment decreased the cholesterol efflux to apolipoprotein A-I(ApoA-I) and high-density lipoprotein(HDL) compared with LDLr-/- mouse lipoprotein treatment. ApoE-/- mouse lipoprotein treatment inhibited the mRNA and protein levels of ABCA1 and ABCG1 compared with LDLr-/- mouse lipoprotein treatment. CONCLUSION:Apolipoprotein E plays an important role in the cholesterol efflux of macrophages, which is associated with its regulatory effect on the expression of ABCA1 and ABCG1. |
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| Keywords: | Atherosclerosis Apolipoprotein E ATP-binding cassette transporter Macrophages Cholesterol |
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