| 发情期蓝狐卵泡发育的显微结构观察(英文) |
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| 引用本文： | 崔凯,田明宇,马泽芳,梁玉颖,李延鹏,高善颂,牛树田,王海涛,梁俊文,杨淑慧.发情期蓝狐卵泡发育的显微结构观察(英文)[J].农业科学与技术,2012(3):644-648,685. |
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| 作者姓名： | 崔凯 田明宇 马泽芳 梁玉颖 李延鹏 高善颂 牛树田 王海涛 梁俊文 杨淑慧 |
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| 作者单位： | 青岛农业大学动物科技学院;东北林业大学野生动物资源学院;山东省动物卫生监督所 |
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| 基金项目： | Supported by Special Fund for Agro-scientific Research in the Public Interest(200903014)~~ |
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| 摘 要： | 目的]对发情期蓝狐卵巢的组织结构和卵泡发育过程的变化进行研究。方法]采集10只发情期蓝狐的左侧卵巢共计10枚,利用光学显微镜对卵巢的组织结构和卵泡的发育过程进行观察,同时利用目镜测微尺和显微照相技术分别对30个原始卵泡、30个初级卵泡、15个生长卵泡、15个囊状卵泡、15个成熟卵泡及各级卵泡中的卵母细胞进行测量和拍照。结果]蓝狐卵巢由生殖上皮、白膜、皮质和髓质构成。皮质内分布着大量的间质细胞及不同发育阶段的卵泡,位于卵巢的外周;髓质位于皮质下层,分布着较多的血管。卵泡发育过程中,各级卵泡和卵母细胞的直径差异较大(卵泡为45.45~974.55μm,卵母细胞为30.23~147.27μm),在初级卵泡阶段出现透明带物质。卵泡闭锁发生于卵泡生长的各个时期,主要表现为卵母细胞的皱缩,细胞核固缩;颗粒细胞松散、退化。结论]为揭示雌性蓝狐的生殖机理提供了组织学依据。
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| 关 键 词： | 发情期 蓝狐 卵巢 卵泡 显微结构 |
Microstructure Observation on the Follicular Development of Blue Fox during the Estrous Period |
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| Kai CUI,Mingyu TIAN,Zefang MA,Yuying LIANG,Yanpeng LI,Shansong GAO,Shutian NIU,Haitao WANG,Junwen LIANG,Shuhui YANG.Microstructure Observation on the Follicular Development of Blue Fox during the Estrous Period[J].Agricultural Science ＆ Technology,2012(3):644-648,685. |
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| Authors: | Kai CUI Mingyu TIAN Zefang MA Yuying LIANG Yanpeng LI Shansong GAO Shutian NIU Haitao WANG Junwen LIANG Shuhui YANG |
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| Institution: | 1.College of Animal Science and Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,China;2.College of Wildlife Resources,Northeast Forestry University,Harbin 150040,China;3.Animal Health Supervision Institute of Shandong Province,Jinan 250022,China |
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| Abstract: | Objective] The ovary microstructure and the changes of the follicle during the development period of blue fox during the estrous period were studied.Method] Ten left ovaries were collected from 10 female blue foxes during the estrous period.The histological structure of the ovary and follicular development process were observed by using optical microscope.And 30 primordial follicles,30 primary follicles,15 growing follicles,15 vesicular follicle,15 mature follicles and the oocytes in the follicles of different developing stages were measured and photographed by eyepiece micrometer and photomicrographic technique respectively.Result] The ovary of blue fox consisted of germinal epithelium,tunica albuginea,cortex and medulla.The cortex,which was located at the periphery of the ovary,contained many interstitial cells and follicles at different developing stages.Medulla was located below the cortex,with a lot of blood vessels.The diameters of follicles at different developmental stages and oocytes had greater differences during the developmental period of follicle.The diameter of follicles was 45.45-974.55 μm and the diameter of oocytes was 30.23-147.27 μm.The zona pellucida appeared at the primary follicle stage.The atresia of follicles occurred at all the developmental stages of follicle and the main manifestations were the shrunk oocyte and karyon,and loose and degraded granular cells.Conclusion] The research provided histological basis for revealing the reproductive mechanism of female blue fox. |
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| Keywords: | Oestrus period Blue fox Ovary Follicle Microstructure |
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