Antioxidant activity of uruguayan propolis. In vitro and cellular assays |
| |
| Authors: | Silva Verónica Genta Gonzalo Möller Matías N Masner Martín Thomson Leonor Romero Natalia Radi Rafael Fernandes Denise C Laurindo Francisco R M Heinzen Horacio Fierro Walter Denicola Ana |
| |
| Institution: | Laboratorio de Fisicoqui?mica Biolo?gica y Enzimologi?a, Instituto de Qui?mica Biolo?gica, Facultad de Ciencias, Universidad de la Repu?blica (UdelaR), Igua? 4225, 11400 Montevideo, Uruguay. |
| |
| Abstract: | The antioxidant capacity of propolis from the southern region of Uruguay was evaluated using in vitro as well as cellular assays. Free radical scavenging capacity was assessed by ORAC, obtaining values significantly higher than those of other natural products (8000 μmol Trolox equiv/g propolis). ORAC values correlated well with total polyphenol content (determined by Folin-Ciocalteu method) and UV absorption. Total polyphenol content (150 mg gallic acid equiv/g propolis) and flavonoids (45 mg quercetin equiv/g propolis) were similar to values reported for southern Brazilian (group 3) and Argentinean propolis. Flavonoid composition determined by RP-HPLC indicates a strong poplar-tree origin. Samples high in polyphenols efficiently inhibit low-density lipoprotein lipoperoxidation and tyrosine nitration. In addition, Uruguayan propolis was found to induce the expression of endothelial nitric oxide synthase and inhibit endothelial NADPH oxidase, suggesting a potential cardiovascular benefit by increasing nitric oxide bioavailability in the endothelium. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
