| 中华绒螯蟹不同组织中基因组DNA含量比较 |
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| 引用本文： | 张代臻,唐伯平,张华彬.中华绒螯蟹不同组织中基因组DNA含量比较[J].水产科学,2009,28(2). |
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| 作者姓名： | 张代臻 唐伯平 张华彬 |
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| 作者单位： | 江苏省滩涂生物资源与环境保护重点建设实验室,盐城师范学院,江苏,盐城,224002 |
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| 基金项目： | 国家自然科学基金，江苏省333工程项目，江苏省高校自然科学重大及面上基础研究项目，盐城师范学院科研课题 |
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| 摘 要： | 用改进后的柱式回收法提取了中华绒螯蟹不同组织中的基因组DNA,并通过紫外吸收光谱法初步分析了附肢肌肉组织、鳃、性腺组织和肝脏组织中基因组DNA的含量,初步得出同质量不同组织中基因组DNA含量大小关系趋向于:鳃>肝脏>性腺>附肢肌肉.
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| 关 键 词： | 中华绒螯蟹 基因组DNA 吸收光谱 |
Genome DNA Concentrations in Different Tissues from Chinese Mitten Crab Eriocheir sinensis |
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| ZHANG Dai-zhen,TANG Bo-ping,ZHANG Hua-bin.Genome DNA Concentrations in Different Tissues from Chinese Mitten Crab Eriocheir sinensis[J].Fisheries Science,2009,28(2). |
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| Authors: | ZHANG Dai-zhen TANG Bo-ping ZHANG Hua-bin |
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| Institution: | ZHANG Dai-zhen,TANG Bo-ping,ZHANG Hua-bin(Jiangsu Provincial Key Laboratory of Coastal Wetl, Bioresources , Environmental Protection,Yancheng Normal University,Yancheng 224002,China) |
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| Abstract: | Total genome DNA concentrations were determined in gills,hepatopancreas,gonad and muscles of Chinese mitten crab Eriocheir sinensis by improved method,and the absorption spectra of the genome DNA in different tissues were analysed by GBS UV/Vis.The results showed that the genome DNA concentrations were arranged in a descendant order as the following: gill> hepatopancreas >gonad>muscle. |
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| Keywords: | Eriocheir sinensis genome DNA absorption spectrum |
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