| 日本柄瘤蚜茧蜂的寄生对黑豆蚜生化代谢的影响 |
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| 引用本文： | 甘明,苗雪霞,丁德诚.日本柄瘤蚜茧蜂的寄生对黑豆蚜生化代谢的影响[J].植物保护学报,2003,30(3):255-260. |
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| 作者姓名： | 甘明 苗雪霞 丁德诚 |
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| 作者单位： | 1. 中国科学院上海生命科学院植物生理生态研究所,上海,200032;中山大学中山医学院寄生虫教研室,广州,510080
2. 中国科学院上海生命科学院植物生理生态研究所,上海,200032 |
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| 基金项目： | 中国科学院院长基金资助 |
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| 摘 要： | 日本柄瘤蚜茧蜂Lysiphlebus japonicus Ashmead的寄生影响了寄主黑豆蚜Aphis craccivora Koch体内的生化代谢。寄生使黑豆蚜的游离氨基酸总浓度升高，寄生1天后，正常组的游离氨基酸总浓度为17．721nmol／L，被寄生组为23．153nmol／L；寄生4天后，正常组和被寄生组分别为58．703和69．659nmol／L。苏氨酸、谷氨酸和酪氨酸是黑豆蚜血淋巴中主要的氨基酸，被寄生后的黑豆蚜血淋巴中苏氨酸含量升高，谷氨酸和酪氨酸含量下降。与正常蚜虫相比，被寄生后3天，黑豆蚜血淋巴的蛋白质浓度下降，体蛋白质浓度升高；寄生还使寄主血淋巴的蛋白质组成发生了变化，在SDS-聚丙烯酰胺凝胶电泳图谱中出现了两个新的蛋白质，其分子量分别约为54和41kD。在被寄生后4天，黑豆蚜的血淋巴海藻糖浓度明显降低。蚜虫的体总糖原和体总脂含量在被寄生后2天显著升高，但在被寄生后3、4天明显低于未寄生蚜虫。
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| 关 键 词： | 日本柄瘤蚜茧蜂 寄生 黑豆蚜 生化代谢 影响因素 游离氨基酸 浓度 寄生组 |
| 收稿时间： | 2002/6/24 0:00:00 |
| 修稿时间： | 2002年6月24日 |
Biochemical and metabolic alterations in Aphis craccivora Koch parasitized by Lysiphlebus japonicus Ashmead |
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| Gan Ming,Miao Xuexia and Ding Decheng.Biochemical and metabolic alterations in Aphis craccivora Koch parasitized by Lysiphlebus japonicus Ashmead[J].Acta Phytophylacica Sinica,2003,30(3):255-260. |
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| Authors: | Gan Ming Miao Xuexia and Ding Decheng |
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| Institution: | Institute of Plant Physiology and Ecology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China;Department of Parasitology, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510080, China,Institute of Plant Physiology and Ecology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China and Institute of Plant Physiology and Ecology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China |
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| Abstract: | Biochemical and metabolic alterations in Aphis craccivora Koch parasitized by Lysiphle-bus japonicus Ashmead are reported. Experiments were carried out on parasitized 2nd instar aphids. Parasitism increased the total free amino acid level in hemolymph, with the Thr, Glu and Tyr as the most dominating relative abundance. On days 1 and 4 after parasitoid oviposition, the free amino acid levels were 23.153nmol/L, 69.659nmol/L respectively. In contrast, that of normal hosts were 17.721nmol/L and 58. 703nmol/L. The increase of Thr and decrease of Glu and Tyr were observed. The hemolymph protein level descended, while the body protein level ascended on 3 days after parasitoid oviposition. PAGE analysis of hemolymph revealed the presence of two parasitism-specific proteins, which of approximately 54 and 41kD. A significant decline in hemolymph tre-halose concentration was determined on 4 days after the aphid being parasitized. The total body glycogen and total body lipid titers were significant higher on 2 days after the aphid being parasitized , then distinct lower than the nomal aphids on 3 and 4 days after parasitoid oviposition. |
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| Keywords: | Aphis craccivora Koch Lysiphlebus japonicus Ashmead parasitism metabolism |
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