| E.tenella初次感染雏鸡外周血T淋巴细胞亚群及增殖能力变化的动态研究 |
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| 引用本文： | 黄海斌,杨桂连.E.tenella初次感染雏鸡外周血T淋巴细胞亚群及增殖能力变化的动态研究[J].中国兽药杂志,2014,48(10):6-10. |
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| 作者姓名： | 黄海斌 杨桂连 |
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| 作者单位： | 吉林农业大学动物科学技术学院 吉林省动物微生态制剂工程研究中心,吉林农业大学动物科学技术学院 吉林省动物微生态制剂工程研究中心 |
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| 基金项目： | 国家“863”计划项目（2013AA102806，2011AA10A215）；国家自然科学基金项目（31272552，31272541，81170358）；教育部新世纪优秀人才支持计划项目（NCET-10-0175）；吉林省科技发展计划项目（20111816）；吉林省世行贷款农产品质量安全项目（2011-Y07） |
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| 摘 要： | 应用流式细胞术（ FCM）和MTS比色法检测雏鸡初次感染柔嫩艾美尔球虫（ E． tenella）后外周血中CD3＋CD4＋、CD3＋CD8＋T淋巴细胞及其增殖能力的动态变化。数据显示，E． tenella初次感染雏鸡后7～20 d CD3＋CD4＋、CD3＋CD8＋T 淋巴细胞比例明显高于对照组雏鸡（ P＜0．05和P＜0．01），并且均于12 d时达到峰值。初次感染后7～20 d雏鸡外周血中CD4＋／CD8＋T淋巴细胞亚群的比值明显升高，于感染后16 d时达到1．78。 E． tenella初次感染雏鸡后12～20 d外周血淋巴细胞增殖能力明显高于对照组雏鸡（ P＜0．05），并于16 d达到峰值。试验表明E． tenella初次感染雏鸡能激活淋巴细胞产生增殖反应，CD3＋CD4＋、CD3＋CD8＋T淋巴细胞在抵抗E． tenella初次感染过程中有重要作用。
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| 关 键 词： | 柔嫩艾美尔球虫 外周血 CD3+CD4+ CD3+CD8+ 增殖 |
| 收稿时间： | 2014/8/12 0:00:00 |
| 修稿时间： | 2014/8/28 0:00:00 |
Studies on Dynamic Changes of T lymphocytes subpopulations and lymphocytes proliferation in Peripheral Blood of Chickens Primary Infected with E.tenella |
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| Institution: | College of Animal Science and Technology,Jilin Agricultural University,Jilin Provincial Engineering Research Center of Animal probiotics,Changchun, |
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| Abstract: | Flow cytometry （ FCM ） and MTS colorimetric assay was used to study dynamic changes of T lymphocyte subpopulations and lymphocytes proliferation in peripheral blood of chicks after primary infection with E.tenella. The results were as follows：the numbers of CD3＋CD4＋and CD3＋CD8＋T lymphocytes in peripheral blood of chicks after primary infection with E. tenella were increased significantly comparing with controls after 7~20 days post infection（ P〈0.05 or P〈0.01） , and the peak appeared at 12th days after primary infection. Peripheral blood the CD4＋/ CD8＋ T lymphocytes ratio in peripheral blood of chicks after primary infection with E. tenella were＆amp;nbsp;significantly increased comparing with controls 7~20 days post infection, the ratio 1.78 at 16th after the infection. The proliferative responses of T lymphocytes to PHA in peripheral blood of chicks infected with E. tenella were significantly elevated compared with that of control chicks at day 12~20 after primary infection, and peaked at days of 16th. The results indicated that the cellular immune was increased after primary infection and CD3＋CD4＋, CD3＋CD8 ＋T lymphocytes play an important role in different period when chicks resisted E. tenella infection. |
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| Keywords: | E tenella peripheral blood CD3 CD4 CD3 CD8 proliferation |
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