| 液相色谱-串联质谱法测定饲料中磺胺类和喹诺酮类药物的含量 |
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| 引用本文： | 彭 丽,吴宁鹏.液相色谱-串联质谱法测定饲料中磺胺类和喹诺酮类药物的含量[J].中国兽药杂志,2014,48(10):53-59. |
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| 作者姓名： | 彭 丽 吴宁鹏 |
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| 作者单位： | 河南省兽药监察所,郑州,450008 |
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| 摘 要： | 建立了同时测定饲料中21种磺胺类药物和13种喹诺酮类药物的超高效液相色谱-串联质谱方法。以2％氨水乙腈和0．1 mol／L 氢氧化钠溶液提取样品中的待测物，HLB固相萃取柱净化，液相色谱-串联质谱法测定，外标法定量。药物在1～50 ng／mL浓度范围内线性关系良好，相关系数均大于0．99。方法的检出限为0．05 mg／kg，定量限为0．10 mg／kg，在不同饲料中添加浓度范围为0．1～100 mg／kg，平均回收率为60．6％～118．6％，相对标准偏差在1．9％～15．2％之间。本方法抗干扰能力强、分析速度快、灵敏度高，适用于饲料中磺胺类和喹诺酮类药物的测定。
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| 关 键 词： | 饲料 磺胺类药物 喹诺酮类药物 超高效液相色谱-串联质谱 |
| 收稿时间： | 2014/7/18 0:00:00 |
| 修稿时间： | 2014/9/10 0:00:00 |
Determination of Sulfonamides and Quinolones in Feeds by Ultra Performance Liquid Chromatography- Tandem Mass Spectrometry |
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| PENG Li
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WU Ning-peng
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ZHANG Fa-wang
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CHEN Qiang
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DU Hong-ge
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LI Hui-su.Determination of Sulfonamides and Quinolones in Feeds by Ultra Performance Liquid Chromatography- Tandem Mass Spectrometry[J].Chinese Journal of Veterinary Drug,2014,48(10):53-59. |
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| Authors: | PENG Li WU Ning-peng ZHANG Fa-wang CHEN Qiang DU Hong-ge LI Hui-su |
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| Institution: | Henan Institute of Veterinary Drug Control, |
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| Abstract: | A method based on ultra performance liquid chromatography-tandem mass spectrometry （ UPLC-MS/MS） has been developed for the determination of sulfonamides and quinolones in feeds. The analyte was extracted with acetonitrile containing 2% ammonia and 0. 1 mol/L sodium hydroxide solution, and cleaned by HLB solid phase extraction cartridge. Quantification of the analyte was achieved by UPLC-MS/MS with multiple reaction monitoring （ MRM ） using external standard method. The calibration curves of the drugs were linear in the concentration ranges of 1 ~ 50 ng/mL with correlation coefficients more than 0. 99. Detection limits and quantification limits of the method for the analyte were 0. 05 mg/kg and 0. 1 mg/kg in feeds. The average recoveries ranged from 60.6% to 118.6% at the spiked level of 0.1~100 mg/kg in feeds. The relative standard deviation was from 1. 9% to 15. 2%. With the advantages of good anti-interference ability, rapidness and sensitivity, the method adapted to the determination of sulfonamides and quinolones in feeds. |
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| Keywords: | UPLC-MS/MS feeds sulfonamides quinolones |
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