| 毛白杨叶片再生系统的建立 |
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| 引用本文： | 杜宁霞,李云,于海武,林善枝,张志毅.毛白杨叶片再生系统的建立[J].中国林学,2002,4(2):48-51. |
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| 作者姓名： | 杜宁霞 李云 于海武 林善枝 张志毅 |
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| 作者单位： | 北京林业大学生物科学与技术学院,北京,100083 |
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| 摘 要： | 该文选用三倍体毛白杨的叶片作为外植体 ,MS作为基本培养基 ,采用不同组合的BA和NAA作为植物生长调节剂对毛白杨再生系统进行研究 .结果表明 ,在MS培养基中单独添加 1 5mg·L-1BA时 ,不定芽分化率为 90 % ,同时伴随一定程度的玻璃化现象 .单独添加 0 3mg·L-1NAA时 ,不定根产生率最高 (90 % ) ,但无不定芽生成 .采用 1 0mg·L-1BA + 0 3mg·L-1NAA组合搭配时 ,不定芽分化率最高 (95 % ) .在此基础上 ,采用M1(MS + 1 0mg·L-1BA + 0 3mg·L-1NAA)培养基 ,研究了不同着生位置的叶片、同一着生位置叶片的不同切段以及叶片在培养基上的放置方式对毛白杨叶片再生的影响 .结果表明带有叶柄的下切段比其余叶片切段不定芽再生率高 ,幼嫩叶片比老化叶片再生率高 ,叶片近轴端向下接触培养基比远轴端向下接触培养基再生率高 .叶片再生系统的建立为毛白杨的遗传转化奠定了基础
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| 关 键 词： | 三倍体毛白杨 叶片外植体 再生 |
Establishment of High Frequency Regeneration System of Populus tomentosa |
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| Du Ningxia,Li Yun,Yu Haiwu,Lin Shanzhi,Zhang Zhiyi.Establishment of High Frequency Regeneration System of Populus tomentosa[J].Forestry Studies in China,2002,4(2):48-51. |
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| Authors: | Du Ningxia Li Yun Yu Haiwu Lin Shanzhi Zhang Zhiyi |
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| Abstract: | The establishment of high frequency regeneration system is a foundation for Agrobacterium- mediated genetic transformation. In this work, several important factors influencing the efficiency of regeneration of plants, such as concentration of plant growth regulators, leaf explant orientation, leaf growth sequence and leaf segment, were studied. The results indicated that the differentiation rate of adventitious shoots was 90 % on basal MS medium only supplemented with 1.5 mg@ L- 1 BA (6-benzyladenine) and reached the highest level (95 % ) when 1.0 mg@L-1 BA and 0.3 mg@L-1NAA (naphthaleneacetic acid) were added to MS medium. 90% of differentiation rate of adventitious roots were obtained when 0.3 mg@ L- 1 NAA was only added to MS medium. It was also found that more adventitious shoots were regenerated from the lower segment of leaf (with petiole) than the other segments, the number of adventitious shoots decreased from top to base of leaf growth sequence and the percentage of adventitious shoot induction with adaxial side downward was higher than that with adaxial side upward. |
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| Keywords: | triploid Populus tomentosa leaf explant regeneration |
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