| 影响菜心游离小孢子培养的因素 |
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| 引用本文： | 朱允华,刘明月,吴朝林.影响菜心游离小孢子培养的因素[J].长江蔬菜,2003(9):46-47. |
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| 作者姓名： | 朱允华 刘明月 吴朝林 |
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| 作者单位： | 1. 湖南农业大学园艺园林学院,长沙,410128
2. 湖南省农科院蔬菜所 |
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| 摘 要： | 对影响菜心游离小孢子培养诱导胚状体的主要因素如基因型、花序低温预处理、高温起始培养和培养基进行了研究，结果表明，基因型对小孢子培养起着决定性的作用，不同材料之间，诱导频率差异显著；低温预处理幼花序可明显提高小孢子胚状体的诱导率，培养基中加入活性炭可改善胚的分化，高温预培养促进胚状体诱导。
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| 关 键 词： | 菜心 游离小孢子培养 基因型 低温预处理 培养温度 培养基 |
Factors to Affect Isolated Microspore Culture in Brassica Parachinensis Bailey |
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| Zhu Yunhua,Liu Mingyue,Wu Chaolin.Factors to Affect Isolated Microspore Culture in Brassica Parachinensis Bailey[J].Journal of Changjiang Vegetables,2003(9):46-47. |
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| Authors: | Zhu Yunhua Liu Mingyue Wu Chaolin |
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| Abstract: | The main factors to affect isolated microspore culture such as genotype,low temperature pretreatment to buds,high temperature shock and medium were studied.The results show that genotype plays a decisive role in isolated mi-crospore culture,the induction frequency of embryoids among different genotypes differs significantly,low temperature pretreat-ment to buds can obviously improve the induction frequency of embryoids,active carbon in the medium and1-2days high temperature at the beginning culture are beneficial to the successful development of cultured microspores into embryos? |
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| Keywords: | Brassica Parachinensis Bailey Microspore Genotype |
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