| 大葱伴生栽培对黄瓜根区土壤细菌种群的影响 |
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| 引用本文： | 夏秀波,王全华,葛晨辉,曹守军,李涛,姚建刚,张瑞清.大葱伴生栽培对黄瓜根区土壤细菌种群的影响[J].中国蔬菜,2013,1(16):72-77. |
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| 作者姓名： | 夏秀波 王全华 葛晨辉 曹守军 李涛 姚建刚 张瑞清 |
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| 作者单位： | （山东省烟台市农业科学研究院，山东烟台 265500） |
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| 基金项目： | 现代农业产业技术体系建设专项(CARS-25-G-25);山东省科技发展计划项目(2012GNC11026) |
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| 摘 要： | 以嫁接黄瓜为试材，采用PCR-DGGE 技术研究了大葱伴生栽培对黄瓜根区土壤细菌种群的
影响。结果表明：大葱伴生栽培对黄瓜根区土壤细菌种群有一定的影响；大葱伴生栽培降低了土壤细菌种
群的多样性指数和均匀度指数；并且随着黄瓜的发育根区土壤细菌多样性也发生了变化。对DGGE 条带进
行测序，结果表明：大葱伴生栽培黄瓜根区土壤细菌种群出现了某些细菌特有的序列，可归为4 个推测的
细菌类群，分别为酸杆菌纲（Acidobacteria）、放线菌纲（Actinobacteria）、鞘脂单胞菌属（Sphingomonas）
和假单胞菌属（Pseudomonadaceae）。
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| 关 键 词： | 大葱 伴生栽培 嫁接黄瓜 土壤细菌 PCR-DGGE |
Effect of Companion Planting Chinese Onion on Soil Bacterial Communities
in Root Zone of Cucumber |
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| XIA Xiu-bo,WANG Quan-hua,GE Chen-hui,CAO Shou-jun,LI Tao,YAO Jian-gang,ZHANG Rui-qing.Effect of Companion Planting Chinese Onion on Soil Bacterial Communities
in Root Zone of Cucumber[J].China Vegetables,2013,1(16):72-77. |
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| Authors: | XIA Xiu-bo WANG Quan-hua GE Chen-hui CAO Shou-jun LI Tao YAO Jian-gang ZHANG Rui-qing |
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| Institution: | （Yantai City Institute of Agricultural Sciences， Yantai 265500， Shandong， China） |
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| Abstract: | By PCR-DGGE method，the paper studies the effect of companion planting Chinese
onion Allium fistulosum L. var. giganteum Makino on the bacterial communities in root zone of grafted
cucumber（Cucumis sativus L.）．The results showed that with test plants companion planting，the
Chinese onion decreased the diversity and evenness indices of bacterial communities in root zone of
grafted cucumber．And the diversity of soil bacterial communities varied with the growing stages of
cucumber．The sequencing of DGGE bands indicated that the characteristic bands of companion planting
Chinese onion were classified as Acidobacteria，Actinobacteria，Sphingomonas and Pseudomonadaceae，
respectively. |
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| Keywords: | Chinese onion Companion planting Grafted cucumber Soil bacterial PCRD-GGE |
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