| 高山被孢霉挥发性次生代谢产物分析 |
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| 引用本文： | 刘欣,马小琛,黄和,于文涛,纪晓俊,彭超,高振.高山被孢霉挥发性次生代谢产物分析[J].安徽农业科学,2009,37(29). |
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| 作者姓名： | 刘欣 马小琛 黄和 于文涛 纪晓俊 彭超 高振 |
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| 作者单位： | 南京工业大学生物与制药工程学院,江苏南京,210009;南京工业大学生物与制药工程学院,江苏南京,210009;江苏省工业生物技术创新中心,江苏南京,211816 |
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| 基金项目： | 国家自然科学基金，973计划，江苏省六大人才高峰资助项目 |
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| 摘 要： | 目的]研究花生四烯酸生产菌高山被孢霉挥发性次生代谢产物成分,并探讨其代谢途径的变化。方法]采用固相微萃取提取高山被孢霉对数生长后期发酵液上方空气并进行GC-MS分析。结果]一共分析得到13种化合物,其中,12种均为C15H24的倍半萜化合物,浓度占总组分的99.62%。含量最高的3个化倍半萜合物Thujopsene-(12)(斧柏烯)、α-Guaiene(愈创木烯)和Aristolene(土青木香烯)分别占到总含量的10.66%、33.69%和34.85%。通过次级代谢产物的检测能够较充分证明高山被孢霉中存在倍半萜的代谢途径。结论]原始菌株经过诱变致使异戊二烯或倍半萜分支途径中一个或几个关键酶的基因发生突变而使其编码的酶活性增强,从而导致此条代谢途径的通量增加而有利于倍半萜化合物的大量积累。
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| 关 键 词： | 高山被孢霉 固相微萃取 倍半萜 花生四烯酸 |
Analysis on the Volatile Secondary Metabolites of Mortierella alpina |
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| LIU Xin et al.Analysis on the Volatile Secondary Metabolites of Mortierella alpina[J].Journal of Anhui Agricultural Sciences,2009,37(29). |
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| Authors: | LIU Xin |
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| Institution: | LIU Xin et al(College of Biotechnology , Pharmaceutical Engineering,Nanjing University of Technology,Nanjing,Jiangsu 210009) |
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| Abstract: | Objective]The study was to investigate the volatile components of secondary metabolites from M.alpine producing arachidonic acid and explore the changes in its metabolic pathway.Method] The air above M.alpine broth was extracted by solid-phase microextraction(SPME) during the post-exponential phase of growth and analyzed by GC-MS.Result] 13 compounds were identified,12 of which were sesquiterpenes with C15H24 formula and accounting for 99.62% of the complete compounds.Thujopsene-(12),α-Guaiene and Aristolene were three most sesquiterpenes accounting for 10.66%,33.69% and 34.85% of total content,respectively.It can be sufficiently certified that sesquiterpene metabolic pathway existing in M.alpine.Conclusion] One or several key enzymes genes mutation enchaned the activities of their encoding enzymes in isoprene or sesquiterpene pathuay after initial strain mutation,which led flux rising of this metabolic pathway and helped mass accumulation of sesquiterpenes. |
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| Keywords: | Mortierella alpina SPME Sesquiterpene Arachidonic acid |
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