Mononuclear phagocyte receptors in rhesus monkeys (Macaca mulatta) and their role in hemolytic disease of the newborn |
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| Authors: | R C Stong W H Stone |
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| Institution: | Laboratory of Genetics and Wisconsin Regional Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53706 USA |
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| Abstract: | Hemolytic disease of the newborn does not develop in rhesus monkeys because placentally-transferred maternal antibodies do not induce immune clearance of the newborn's erythrocytes. In an in vitro RBC adherence assay, rhesus peripheral blood monocytes did not bind newborn's RBC which had been coated in utero or in vitro with maternal antibodies. Nevertheless, rhesus phagocytes possess receptors that are specific for the Fc portion of IgC and for the C3b. Using purified human IgG subclasses as inhibitors of RBC adherence, rhesus Fc receptors preferentially bind IgG1 and IgG3. Thus, it may be that maternal antibodies are non-opsonic because they belong to IgG subclasses that do not bind effectively to rhesus Fc receptors. Also, RBC adherence appears to be controlled by the level of antibody coating which in turn is determined by avidity of the antibodies and by the number of RBC membrane determinants. The failure of maternal antibodies to opsonize the newborn's RBC and thus cause hemolytic disease is very likely due to the low avidity of antibodies and to the weak expression of blood group determinants on the membranes of these RBC. |
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| Keywords: | DAT direct antiglobulin agglutination test FCS fetal calf serum HDN hemolytic disease of the newborn HBSS Ig immunoglobulin PBM peripheral blood monocytes PBS phosphate buffered saline RBC erythrocyte(s) RES reticuloendothelial system IS alloimmune serum MS maternal postpartum serum |
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