| 抗菌肽Lactoferricin基因的PCR合成及克隆载体T-Lfn的构建 |
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| 引用本文： | 李铁晶,黄占权,许岩.抗菌肽Lactoferricin基因的PCR合成及克隆载体T-Lfn的构建[J].东北农业大学学报,2008,39(1):107-111. |
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| 作者姓名： | 李铁晶 黄占权 许岩 |
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| 作者单位： | 东北农业大学食品学院,哈尔滨,150030 |
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| 基金项目： | 黑龙江省杰出青年科学基金 |
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| 摘 要： | Lactoferricin是一种天然抗菌肽。试验进行了Lactoferricin基因的PCR合成,将其引入质粒,转化E.coliJM-109,并进行PCR鉴定、酶切鉴定及测序,成功构建了Lactoferricin基因克隆载体T-Lfn。
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| 关 键 词： | 抗菌肽 乳铁素 载体构建 |
| 文章编号： | 1005-9369（2008）01-0107-05 |
| 收稿时间： | 2006-08-24 |
| 修稿时间： | 2006年8月24日 |
PCR synthesization and construction of cloning vector T-Lfn of antimicrobial peptide Lactoferricin gene |
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| LI Tiejing,HUANG Zhanquan,XU Yan.PCR synthesization and construction of cloning vector T-Lfn of antimicrobial peptide Lactoferricin gene[J].Journal of Northeast Agricultural University,2008,39(1):107-111. |
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| Authors: | LI Tiejing HUANG Zhanquan XU Yan |
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| Abstract: | Lactoferricin is a kind of natural antibacterial peptide. In this study, the Lactoferricin gene was synthesized with PCR, linked with the plasmid of pMD18-T Vector, and transformed into E.coli JM-109. The identifications of PCR, enzymical degestion and sequencing were carried out, and the cloning vector of T-Lfn was successfully constructed. |
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| Keywords: | antimicrobial peptide Lactoferricin vector construction |
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