| RAPD-PCR技术在根结线虫分类鉴定中的应用 |
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| 引用本文： | 白万明,吴建宇.RAPD-PCR技术在根结线虫分类鉴定中的应用[J].云南农业大学学报,1996,11(4):216-221. |
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| 作者姓名： | 白万明 吴建宇 |
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| 作者单位： | [1]云南农业大学植物病理系 [2]云南省植物病理重点实验室 |
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| 摘 要： | 采用Operon公司A组的9个引物对3种根结线虫:南方根结线虫1号小种(Meloidogyne incognitarace 1),爪哇根结线虫(M.javanica),北方根结线虫(M.hapla)的基因组DNA进行随机扩增,扩增产物显示了明显的多态性,其中引物OPA-01、OPA-03、OPA-04、OPA-05能区分3种根结线虫,其它5个引物仅能区分1个或2个种.
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| 关 键 词： | 根结线虫 RAPD-PCR 分类鉴定 |
| 收稿时间： | 1996-10-03 |
Application of RAPD-PCR Technology in Identification of Three Major Meloidogyne spp. |
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| Bai Wanming,WuJianyu,Hu Xianqi,Yu Shengfu.Application of RAPD-PCR Technology in Identification of Three Major Meloidogyne spp.[J].Journal of Yunnan Agricultural University,1996,11(4):216-221. |
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| Authors: | Bai Wanming WuJianyu Hu Xianqi Yu Shengfu |
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| Institution: | Department of Plant Pathology, Yunnan Agricultural University, Phytopathology Laboratory of Yunnan Province, Kunming 650201 |
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| Abstract: | In this paper,Nine primers(from Operon Technologies)were evaluated for their usefulness in identifying the 3 major Meloidogyne spp:M. incognita race 1,M. javanica,M. hapla. The amplified products show numerous polymorphisms.Reaction with primer OPA-01,OPA-03,OAP-04,OAP-05 can produce amplified DNA bands whose size allow the seperation of the 3 species,and reation with other 5 primers can only seperate one or two species of them. |
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| Keywords: | Meloidogyne spp Meloidogyne spp" target="_blank">')" href="#">Meloidogyne spp RAPD-PCR Identification |
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