Diagnostic utility of measuring serum amyloid A with a latex agglutination
turbidimetric immunoassay in bovine mastitis: Comparison with haptoglobin and alpha 1 acid
glycoprotein |
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| Authors: | Marina OTSUKA Mieko SUGIYAMA Takaaki ITO Kenji TSUKANO Shin OIKAWA Kazuyuki SUZUKI |
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| Institution: | 1)School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan;2)Ehime Agricultural Mutual Aid Association, 4-4-2 Nibancho, Matsuyama, Ehime 790-0002, Japan;3)Aichi Agricultural Mutual Aid Association, 2-1-11 Marunouchi, Nakaku, Nagoya 460-0002, Japan;4)Minami-Hokkaido Agricultural Mutual Aid Association, 74-2 Higashimae, Hokuto, Hokkaido 041-1214, Japan |
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| Abstract: | This study established the precision and accuracy of a modified latex agglutination turbidimetric immunoassay (LATIA) reagent, and evaluated the ability of the measurement of serum amyloid A (SAA) compared to haptoglobin and α1-acid glycoprotein, which are acute phase proteins (APPs), for diagnosis of clinical mastitis. Concentrations of APPs in cows with mastitis were significantly higher than those in healthy cow. Only the plasma SAA concentration in cows with clinical mastitis (44.90 mg/l; n=15) was significantly higher than that in those with subclinical mastitis (10.70 mg/l; n=16), enabling their diagnosis in contrast to other APPs. Thus, the SAA assay using a LATIA reagent is useful in assessing mastitis severity due to its higher sensitivity and specificity than other APP assays. |
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| Keywords: | acute phase protein bovine inflammation mastitis serum amyloid A |
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