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| 反相高效液相色谱法测定小鼠心肌中ATP、ADP和AMP含量及分析 | |
| 引用本文： | 孙蕊,贾鹏禹,俞龙浩,何淑清,张盟.反相高效液相色谱法测定小鼠心肌中ATP、ADP和AMP含量及分析[J].黑龙江八一农垦大学学报,2012,24(2):30-32,57. |
| 作者姓名： | 孙蕊 贾鹏禹 俞龙浩 何淑清 张盟 |
| 作者单位： | 1. 黑龙江八一农垦大学动物科技学院,大庆,163319 2. 黑龙江省农产品加工工程技术研究中心 3. 黑龙江八一农垦大学食品学院 |
| 摘 要： | 建立同时测定小鼠心肌组织中ATP（三磷酸腺苷）、ADP（二磷酸腺苷）和AMP（一磷酸腺苷）含量的高效液相色谱方法。采用反相高效液相色谱模式，使用SepaxBio-C18（250ram×4．6mmID，μm，200A）色谱柱，以60mmoL·L^-1磷酸二氢钾、60mmoL·L^-1磷酸氢二钾组成的缓冲液（pH=6．68）为流动相等比洗脱，流速：O．6mL·min^-1紫外检测波长为259Nm，带宽为30nm。三种目标组分在10min内实现基线分离，平均加标回收率均在99％以上，线性范围为1-200mg·L^-1检出限分别为20、20、25ng。该法简便、准确，易于移植。 |
| 关 键 词： | 高效液相色谱 ATP ADP AMP 心肌 小鼠 |
Determination of ATP, ADP and AMP in Rat Myocardium by RP-HPLC |
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| Sun Rui,Jia Pengyu,Yu Longhao,He Shuqing,Zhang Meng.Determination of ATP, ADP and AMP in Rat Myocardium by RP-HPLC[J].Journal of Heilongjiang August First Land Reclamation University,2012,24(2):30-32,57. | |
| Authors: | Sun Rui Jia Pengyu Yu Longhao He Shuqing Zhang Meng |
| Institution: | 1.College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,Daqing 163319; 2.Agri-Food Processing and Engineering Technology Research Center of Heilongjiang Province; 3.College of Food Science,Heilongjiang Bayi Agricultural University) |
| Abstract: | To establish the simultaneous determination of the content of ATP,ADP and AMP in rat myocardial tissue,HPLC was adopted.It was determined by RP-HPLC with a Sepax Bio-C18(250 mm×4.6 mm ID,5 μm,200 )column,and UV detector at a wave length of 259 nm(BW 30 nm).Mobile phase was 60 mmol·L-1 KH2PO4 and 60 mmol·L-1 K2HPO4(pH=6.68) and flow rate was 0.6 mL·min-1.The separation was achieved within 10 min.All the calibration curves were linear in the mass concentration range of 1~200 mg·L-1.The average recoveries of the standard additions were over 97%,and the LOD were 20,20 and 25 ng for ATP,ADP and AMP respectively.This method was proved to be reliable and reproducible. |
| Keywords: | HPLC ATP ADP AMP myocardium rat |
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