| 植物生长调节剂对羽衣甘蓝小孢子胚发生的影响 |
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| 引用本文： | 姜凤英,冯辉.植物生长调节剂对羽衣甘蓝小孢子胚发生的影响[J].园艺学报,2006,33(3):642-644. |
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| 作者姓名： | 姜凤英 冯辉 |
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| 作者单位： | (沈阳农业大学园艺学院, 辽宁沈阳110161; 辽宁省农业科学院花卉研究所, 辽宁沈阳110161) |
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| 摘 要： | 以‘圆叶红心’和‘皱叶红心’2个羽衣甘蓝F1杂交种为试材, 进行游离小孢子培养, 研究了6-BA、NAA和2, 4-D对其胚状体诱导的影响, 结果表明: 6-BA对诱导胚状体起促进作用, 其适宜浓度为0.2 mg·L - 1 ; NAA抑制小孢子胚状体发生, 但能促进其发育, 提高正常胚比例; 2, 4-D有较强的抑制作用, 只有少量细胞团的形成; 6-BA、NAA综合作用能显著提高胚状体诱导率, 其浓度配比以1∶1或2∶1为佳。
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| 关 键 词： | 羽衣甘蓝 小孢子培养 胚状体 生长素 细胞分裂素 |
| 文章编号： | 0513-353（2006）03-0642-03 |
| 收稿时间： | 2005-07-25 |
| 修稿时间： | 2005-07-252005-10-20 |
Effect of the Auxin and Ctytokin on the Frequency of Embryogenesis in Kale |
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| Jiang Fengying,Feng Hui.Effect of the Auxin and Ctytokin on the Frequency of Embryogenesis in Kale[J].Acta Horticulturae Sinica,2006,33(3):642-644. |
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| Authors: | Jiang Fengying Feng Hui |
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| Institution: | ( College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110161, China; Floriculture Institute of Liaoning Academy of Agricultural Science, Shenyang, Liaoning 110161, China) |
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| Abstract: | The effects of 6-BA, NAA and 2, 4-D on embryoid induction during adopting isolated microspore culture of kale (Brassica oleracea L. var. acephala DC. ) were studied using two hybrids‘Yuanye Hongxin’and ‘Zhouye Hongxin’. The results showed that 6-BA could promote embryoid induction and the suitable content was 0.2 mg·L - 1. NAA could not p romote embryoid formation, but increased the proportion of normal embryoid. 2, 4-D has significant inhibition to embryoid formation and only produce a little agglomerate cells. In addition, the media with 62BA and NAA dramatically increased the frequency of microspore-derived embryoids and the suitable content ration was 1∶1 or 2∶1. |
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| Keywords: | Kale Microspore culture Embryoid Auxin Ctytokin |
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