| 木榄Na~+/H~+逆向转运蛋白基因的克隆 |
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| 引用本文： | 郭庆水,徐立新,袁潜华,于伟.木榄Na~+/H~+逆向转运蛋白基因的克隆[J].热带生物学报,2010,1(2):105-109. |
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| 作者姓名： | 郭庆水 徐立新 袁潜华 于伟 |
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| 作者单位： | 海南大学,农学院,海南,海口,570228 |
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| 摘 要： | 根据北滨藜、胡杨、毛白杨、番杏、盐爪爪、盐角草等已发表的逆向转运蛋白的保守区设计简并引物,通过RT-PCR和RACE技术,从木榄(Bruguiera gymnorrhiza)中克隆Na+/H+逆转运蛋白长度为2130bp的cDNA全长序列,开放阅读框1626bp,编码541个氨基酸和一个终止子,含10个跨膜区。蛋白序列同源分析表明它与蓖麻、胡杨、葡萄、百脉根等植物的液泡膜型Na+/H+逆向转运蛋白序列具有很高的同源性(80%以上),表明该序列确属Na+/H+逆转运蛋白家族的基因,在细胞中起到Na+区隔化作用。
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| 关 键 词： | 木榄 BgNHX1 Na^＋／H^＋逆转运蛋白 同源 跨膜 |
Cloning of Na+ /H+ Antiporter Gene from Bruguiera gymnorrhiza(L.)LAM |
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| GUO Qing-shui,XU Li-xin,YUAN Qian-hua,YU Wei.Cloning of Na+ /H+ Antiporter Gene from Bruguiera gymnorrhiza(L.)LAM[J].Journal of Tropical Biology,2010,1(2):105-109. |
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| Authors: | GUO Qing-shui XU Li-xin YUAN Qian-hua YU Wei |
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| Institution: | GUO Qing-shui,XU Li-xin,YUAN Qian-hua,YU Wei(Agricultural College,Hainan University,Haikou 570228,China) |
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| Abstract: | According to the homologous sequences from Atriplex gmelini,Populus euphratica,Tetragonia tetragonioides,Kalidium foliatum,Populus tomentosa and Salicornia europ,PCR primers were designed and RT-PCR and RACE were used to amplify NHX1 DNA fragment from the leaves of Bruguiera gymnorrhiza.The results indicated that the BgNHX1 contains 2 130 bp,including an open reading frame of 1 626 bp,which encodes a predicted polypeptide of 541 amino acids,and there are 12 predicted transmembrane domains.Base on the data t... |
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| Keywords: | BgNHX1 |
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