| 毛头鬼伞中一条新28S rRNA的发现及其同源性分析 |
| |
| 引用本文： | 王学仁,贾敬芬,邱军兴,张改娜,何涛,郝建国.毛头鬼伞中一条新28S rRNA的发现及其同源性分析[J].广西农业生物科学,2010(1):37-45. |
| |
| 作者姓名： | 王学仁 贾敬芬 邱军兴 张改娜 何涛 郝建国 |
| |
| 作者单位： | 西北大学生命科学学院;陕西省生物技术重点实验室;西安文理学院生命科学系; |
| |
| 基金项目： | 国家自然科学基金(0671087);;西安市科技创新支撑计划(YF07109);;陕西省教育厅科学研究计划项目(06JK203)共同资助 |
| |
| 摘 要： | 本研究从担子菌毛头鬼伞（Coprinus comatus）菌丝中分离获得一条新的28S rRNA序列,序列长度为906bp（GenBank accession No.GU568178）。该序列是我们前期在从毛头鬼伞中克隆一种烟草花叶病毒（TMV）的抗性蛋白基因y3时意外获得的一条非目的条带。将此获得的序列通过NCBI的BLAST,以及与其同源序列进行Clustal w和MEGA聚类分析,证实该序列是28S rRNA,同时还发现毛头鬼伞的系统进化关系比较离散。此外,在这一新28S rRNA与TMV的抗性蛋白基因y3之间发现有两个同源区段有可能是PCR扩增y3基因时出现非目的条带的原因。在这两个同源区段中,其一区段与克隆y3基因时所用的PCR引物之一有较高的相似性,另一区段也是一般PCR引物的类似物。本研究中新28S rRNA序列的获得是PCR扩增中出现非目的条带的新例,该序列的发现及聚类分析的结果有助于真菌基因组学研究及真菌生物分子分类系统的建立。
|
| 关 键 词： | 毛头鬼伞 28S rRNA 聚类分析 |
Finding and Homological Analysis of a New 28S rRNA Sequence Isolated from Shaggy Mane (Coprinus comatus) |
| |
| Wang Xueren , Jia Jingfen Qiu Junxing Zhang Gaina He Tao Hao Jianguo The Provincial Key Laboratory of Biotechnology,School of Life Science,Northwest University,Xi'an,.Finding and Homological Analysis of a New 28S rRNA Sequence Isolated from Shaggy Mane (Coprinus comatus)[J].Journal of Guangxi Agricultural and Biological Science,2010(1):37-45. |
| |
| Authors: | Wang Xueren Jia Jingfen Qiu Junxing Zhang Gaina He Tao Hao Jianguo The Provincial Key Laboratory of Biotechnology School of Life Science Northwest University Xi'an |
| |
| Institution: | Wang Xueren 1,2 Jia Jingfen 1 Qiu Junxing 2 Zhang Gaina 1 He Tao 1 Hao Jianguo 1 1 The Provincial Key Laboratory of Biotechnology,School of Life Science,Northwest University,Xi'an,710069,2 Department of Life Science,Xi'an University of Arts , Science,710065 |
| |
| Abstract: | In this study,we have found a new 28S rRNA sequence with 906 bp in length (GenBank accession No. GU568178) which isolated from mycelia of basidiomycete shaggy mane (Coprinus comatus),and the sequence was an untargeted band obtained unexpectedly when we previously cloned TMV-inhibitor y3 gene from this shaggy mane. The acquired sequence was used to search homologous sequences by using BLAST algorithm in NCBI,and it was performed to cluster with its homologues by using the Clustal w and MEGA methods. The resu... |
| |
| Keywords: | Coprinus comatus 28S rRNA Cluster analysis |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
