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| 内生生防菌P1抑菌物质对马铃薯环腐病菌的抗性及鉴定 | |
| 引用本文： | 王瑞霞,田宏先,杜珍,刘璋,张东旭,张巽.内生生防菌P1抑菌物质对马铃薯环腐病菌的抗性及鉴定[J].山西农业科学,2011,39(4):348-351. |
| 作者姓名： | 王瑞霞 田宏先 杜珍 刘璋 张东旭 张巽 |
| 作者单位： | 1. 山西省农业科学院高寒区作物研究所,山西大同037008;大同大学农学院,山西大同037009 2. 山西省农业科学院高寒区作物研究所,山西大同,037008 3. 大同大学农学院,山西大同,037009 |
| 基金项目： | 山西省科技攻关项目(981014); 山西省农业科学院生物菌剂新产品开发研制项目(2005YCP0501) |
| 摘 要： | 通过平板对峙法发现菌株P1对马铃薯环腐病菌有很强的抑制作用。经分析,抑菌成分是蛋白质,忍受温度80℃,活性成分在280 nm处有最大吸收峰,分子质量大于10 kDa;通过对菌株形态、生理生化特征以及16S rDNA序列系统发育树分析发现,菌株P1与巨大芽孢杆菌相似度达到100%,确定P1菌株为巨大芽孢杆菌(B.megatherium)。电泳图谱检测该序列由1 391个碱基构成,已在GenBank登录(登录号:FJ823003.1)。 |
| 关 键 词： | 马铃薯环腐病 内生生防菌 巨大芽孢杆菌 抗性 鉴定 |
Resistance and Identification of the Endogenous Biocontrol Strain P1 Bacteriostasis Substance against Tomato Bacteria Ring Rot |
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| WANG Rui-xia,TIAN Hong-xian,DU Zhen,LIU Zhang,ZHANG Dong-xu,ZHANG Xun.Resistance and Identification of the Endogenous Biocontrol Strain P1 Bacteriostasis Substance against Tomato Bacteria Ring Rot[J].Journal of Shanxi Agricultural Sciences,2011,39(4):348-351. | |
| Authors: | WANG Rui-xia TIAN Hong-xian DU Zhen LIU Zhang ZHANG Dong-xu ZHANG Xun |
| Institution: | WANG Rui-xia1,2,TIAN Hong-xian1,DU Zhen1,LIU Zhang1,ZHANG Dong-xu2,ZHANG Xun2(1.Institute of the Crops in High Latitude & Cold Climate Area,Shanxi Academy of Agricultural Sciences,Datong 037008,China,2.College of Agronomy,Datong University,Datong 037009,China) |
| Abstract: | Potato ring rot bacteria caused by Clavibacter michiganense subsp.sepedonicum is one of the most important plant diseases.The aim of the experiment is to screen high active antibacterial strain to control this disease and to study the antibacterial substance characteristics.The strain was identified based on the bacteriological characteristics and the 16S rDNA sequence.The culture conditions of P1 strain were optimized by single factor and orthogonal experiment.The strain was identified as Bacillus megather... |
| Keywords: | Potato ring rot endophytic biocontrol bacteria B megatherium resistance identification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |