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Predicting Jatropha curcas seed-oil content, oil composition and protein content using near-infrared spectroscopy—A quick and non-destructive method
Authors:Yiftach Vaknin  Miriam GhanimShahar Samra  Levana DvashEfrat Hendelsman  Dan EisikowitchYael Samocha
Institution:a Department of Agronomy and Natural Resources, Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
b Department of Plant Sciences, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv, Israel
Abstract:The potential of near-infrared reflectance spectroscopy (NIRS) to estimate the oil content, fatty acid composition, and protein content of Jatropha curcas seeds was studied. Seventy-four intact kernels from various sources were scanned by NIRS. All samples were analyzed for oil content (hexane extractions), fatty acid composition (gas chromatography), and protein content (Kjeldahl). Calibration equations were developed for oil content, individual fatty acids (oleic C18:1, linoleic C18:2, stearic C18:0 and palmitic C16:0), and protein content. The performance of the calibration equations was evaluated through external and cross-validation. The results showed that NIRS was a reliable, accurate and nondestructive technique to estimate oil and protein contents, as well as oleic and linoleic fatty acid concentrations in J. curcas kernels; NIRS provides a rapid, simple, and cost-effective alternative method for screening intact J. curcas kernels.
Keywords:Biodiesel  Jatropha curcas  Oil content  Protein content  Fatty acid composition  NIRS
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