| 野桑蚕和不同家蚕品系幼虫体内1-脱氧野尻霉素含量测定 |
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| 引用本文： | 沈以红,朱见,代方银,李竞,向仲怀.野桑蚕和不同家蚕品系幼虫体内1-脱氧野尻霉素含量测定[J].蚕业科学,2007,33(4):674-677. |
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| 作者姓名： | 沈以红 朱见 代方银 李竞 向仲怀 |
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| 作者单位： | 农业部蚕桑学重点开放实验室,重庆,400716;农业部蚕桑学重点开放实验室,重庆,400716;农业部蚕桑学重点开放实验室,重庆,400716 |
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| 摘 要： | 采用反相高效液相色谱(RP-HPLC)-紫外检测法测定了野桑蚕和不同品系家蚕体内的1-脱氧野尻霉素(1-DNJ)含量,并调查了家蚕5龄幼虫不同组织不同生长时期1-DNJ含量的变化。野桑蚕与家蚕、家蚕不同品系间的1-DNJ含量存在极显著差异:野桑蚕全蚕粉中1-DNJ含量最高,质量分数平均为0.427 8%;家蚕以黄血蚕最低,平均0.220 5%。家蚕5龄幼虫不同组织的1-DNJ含量也存在明显差异:家蚕血干粉中的1-DNJ含量最高,质量分数达0.848 7%;其次是中肠、体壁和脂肪体组织,分别为0.512 2%、0.472 2%、0.308 5%;而在丝腺中几乎检测不出1-DNJ。家蚕5龄幼虫体内1-DNJ含量随发育时期呈明显变化,5龄第4天含量最高,达0.329%,第5天以后迅速下降。
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| 关 键 词： | 家蚕 野桑蚕 1-脱氧野尻霉素 反相高效液相色谱-紫外检测法 |
| 文章编号： | 0257-4799（2007）04-0674-04 |
| 收稿时间： | 2007-07-19 |
| 修稿时间： | 2007年7月19日 |
Investigation of 1-DNJ Contents in the Larvae of Bombyx mori and Bombyx mandarina |
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| SHEN Yi-Hong,ZHU Jian,DAI Fang-Ying,LI Jing,XIANG Zhong-Huai.Investigation of 1-DNJ Contents in the Larvae of Bombyx mori and Bombyx mandarina[J].Acta Sericologica Sinica,2007,33(4):674-677. |
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| Authors: | SHEN Yi-Hong ZHU Jian DAI Fang-Ying LI Jing XIANG Zhong-Huai |
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| Abstract: | In this paper,the 1-deoxynojirimycin(1-DNJ) contents in the 5th instar larvae at different developmental stages and tissues of Bombyx mori and Bombyx mandarina were investigated by Reverse-phase High-performance Liquid Chromatography.The results showed that there were obvious 1-DNJ changes in larvae between Bombyx mori and Bombyx mandarina,as well as among various Bombyx mori strains.The highest 1-DNJ content was found in Bombyx mandarina larvae and the average is 0.427 8%.The domesticated silkworm with yellow blood has the lowest 1-DNJ content,and the average level is about 0.220 5%.The 1-DNJ content of translucent silkworm larvae is extremely higher than that of the normal one,and the normal is higher than that with yellow blood.The differences of 1-DNJ content among different tissues are obvious.The highest is in the blood,the next is in the midgut,fat body,and skin,in order.The silkgland almost has no 1-DNJ.The 1-DNJ content of domesticated silkworm larvae changes significantly with developmental stages.It reached a peak of 0.329% at the 4th day of the 5th instar and then decreased quickly. |
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| Keywords: | Bombyx mori Bombyx mandarina 1-Deoxynojirimycin Reverse-phase high-performance liquid chromatography(RP-HPLC) |
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