Relationship Between Resistance Gene Analogue and Blast Resistance in Rice |
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| Authors: | CHEN Yu-min FAN Cheng-ming YANG Yan HE Yue-qiu |
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| Institution: | 1. Key Laboratory of Agricultural Biodiversity for Pest Management of China Education Ministry, Yunnan Agricultural University, Kunming 650201, China;2. College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China;3. Quality Standard and Testing Technology Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China;4. Institute of Agricultural Economy & Information, Yunnan Academy of Agricultural Sciences, Kunming 650205, China |
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| Abstract: | DNA fragments of 43 rice varieties were amplified with 11 pairs of primers designed based on resistance gene analogue (RGA) of plants, and the blast resistance of the varieties was identified by inoculation with 33 isolates of Magnaporthe grisea collected from Yunnan Province, China. Clustering results revealed a significant correlation between the blast resistance and DNA bands with a correlation coefficient of 0.6117 (α=0.01), indicating that the resistance analysis based on RGA-PCR clustering analysis coincided with that based on inoculation. The correlation coefficients, ranging from 0.1701 to 0.535, however, depended on the primers. Five pairs of primers, S1/AS3, S1 INV/S2 INV, XLRR For/XLRR Rev, Pto-Kin1 IN/Pto-Kin2 IN, and NLRR For/NLRR Rev might be applied for blast resistance identification in consideration of their band numbers and polymorphisms, and their correlation coefficients with blast resistance were 0.5305, 0.4898, 0.4059, 0.3719 and 0.3524, respectively. Besides, indica and japonica rice except two highly susceptible varieties, CO39 and Lijiangxintuanheigu, could be well classified by the 11 pairs of primers. |
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| Keywords: | rice resistance gene analogue blast resistance correlation primer inoculation identification |
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