Development of a Cell Culture Method for Quantal Assay of Strain I-2 of Newcastle Disease Virus |
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| Authors: | P Wambura J Meers P Spradbrow |
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| Institution: | (1) School of Veterinary Science, Faculty of Natural Resources, Agriculture and Veterinary Science, University of Queensland, Brisbane, Queensland, Australia |
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| Abstract: | Repeated titrations of strains of Newcastle disease virus (NDV) are more conveniently undertaken in cell cultures rather than
in embryonated eggs. This is relatively easy with mesogenic and velogenic strains that are cytopathic to various cell lines,
but is difficult with avirulent Australian isolates that are poorly cytopathic. Strain V4 for example has been shown to be
pathogenic iin vitro only to of chicken embryo liver cells. Strain I-2 was reported to produce cytopathic effect (CPE) on
chicken embryo kidney (CEK) cells. The present studies confirmed this observation and developed a quantal assay. CEK cells
infected with strain I-2 developed CPE characterized by degeneration, rounding, granularity and vacuolation, and the formation
of synctia. End points were readily established by microscopic examination of fixed and stained cells. In virus infectivity
studies on strain I-2, where multiple titrations are required and where large numbers of samples are used, titration using
CEK cell grown in microtitre plates is recommended. Such studies may not be feasible in embryonated eggs. |
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| Keywords: | cell culture Newcastle disease virus quantal assay strain I-2 vaccine |
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